Metabolic profiling of endogenous bile acids: a novel method to assess hepatoprotective effect of Tanreqing capsule on carbon-tetrachloride-induced liver injury in rats.
10.1016/S1875-5364(18)30057-8
- Author:
Ying XU
1
;
Ping-Ping ZHONG
2
;
Yan-Yan TAO
3
,
4
Author Information
1. Shanghai Kaibao Pharmaceutical Co., Ltd., Shanghai 201401, China. Electronic address: skyxu_1983@163.com.
2. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
3. Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
4. Shanghai Clinical Key Laboratory of Traditional Chinese Medicine, Shanghai 201203, China.
- Publication Type:Journal Article
- Keywords:
Bile acids;
Hepatoprotective effect;
Metabolic profiling;
Tanreqing capsule;
UPLC-MS
- MeSH:
Alanine Transaminase;
blood;
Animals;
Aspartate Aminotransferases;
blood;
Bile Acids and Salts;
blood;
metabolism;
Biomarkers;
blood;
Carbon Tetrachloride;
pharmacology;
Chemical and Drug Induced Liver Injury;
drug therapy;
metabolism;
pathology;
Chromatography, Liquid;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
therapeutic use;
Female;
Liver;
drug effects;
pathology;
Male;
Mass Spectrometry;
Metabolome;
drug effects;
Metabolomics;
Rats;
Rats, Wistar;
Taurocholic Acid;
blood;
Taurodeoxycholic Acid;
analogs & derivatives;
blood
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(4):271-283
- CountryChina
- Language:English
-
Abstract:
Tanreqing (TRQ), a traditional Chinese medicine (TCM) formula, can alleviate liver injury and improve liver function. Its pharmacological mechanisms of actions are still unclear due to its complex components and multi-target natures. Metabolomic study is an effective approach to investigating drug pharmacological actions, new diagnostic markers, and potential mechanisms of actions. In the present study, a new strategy was used to evaluate the protective effect of TRQ capsule against carbon tetrachloride (CCl)-induced hepatotoxicity in rats, by analyzing metabolic profiling of endogenous bile acids (BAs) along with biochemical and histological analyses. BAs concentrations were determined by ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS). Principal component analysis and partial least squares discriminant analysis were then employed to analyze the UPLC-MS results and compare the hepatoprotective effect of TRQ capsule in different groups at the doses of 0.36, 1.44, and 2.88 g·kg body weight, respectively. Moreover, our results suggested that taurocholic acid (TCA) and taurohyodesoxycholic acid (THDCA) were the most important biochemical markers, which were indicative of CCl-induced acute hepatic damage and hepatoprotective effect of TRQ capsule. Therefore, this new strategy would be an excellent alternative method for evaluating hepatoprotective effect and proposing potential mechanisms of action for other drugs as well.
