Osthole decreases collagen I/III contents and their ratio in TGF-β1-overexpressed mouse cardiac fibroblasts through regulating the TGF-β/Smad signaling pathway.
10.1016/S1875-5364(18)30063-3
- Author:
Jin-Cheng LIU
1
,
2
;
Lei ZHOU
3
;
Feng WANG
4
;
Zong-Qi CHENG
5
;
Chen RONG
6
Author Information
1. Clinic Pharmacology Laboratory, Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
2. Department of Pharmacology, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
3. Laboratory Department, Wuxi Center for Disease Control and Prevention, Wuxi 214023, China.
4. Department of Pharmacology, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
5. Clinic Pharmacology Laboratory, Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
6. Clinic Pharmacology Laboratory, Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: rongchen_76@aliyun.com.
- Publication Type:Journal Article
- Keywords:
Collagen;
Mouse cardiac fibroblasts;
Osthole;
TGF-β1
- MeSH:
Actins;
genetics;
Animals;
Cells, Cultured;
Collagen;
biosynthesis;
genetics;
Coumarins;
pharmacology;
Fibroblasts;
drug effects;
metabolism;
Gene Expression Regulation;
drug effects;
Mice;
Myocardium;
cytology;
Protein-Serine-Threonine Kinases;
genetics;
RNA, Messenger;
genetics;
Real-Time Polymerase Chain Reaction;
Receptor, Transforming Growth Factor-beta Type I;
Receptors, Transforming Growth Factor beta;
genetics;
Signal Transduction;
drug effects;
Smad Proteins;
genetics;
Transforming Growth Factor beta1;
genetics
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(5):321-329
- CountryChina
- Language:English
-
Abstract:
The present study was designed to elucidate whether the mechanism by which osthole decreases collagenI/III contents and their ratio is regulating the TGF-β/Smad signaling pathway in TGF-β1-overexpressed mouse cardiac fibroblasts (CFs). These CFs were cultured and treated with different concentrations of osthole. Our results showed that the TGF-β1 expression in the CFs transfected with that the recombinant expression plasmids pcDNA3.1(+)-TGF-β1 was significantly enhanced. After the CFs were treated with 1.25-5 μg·mL of osthole for 24 h, the mRNA and protein expression levels of collagensIand III were reduced. The collagen I/III ratio was also reduced. The mRNA and protein expression levels of TGF-β1, TβRI, Smad2/3, P-Smad2/3, Smad4, and α-SMA were decreased, whereas the expression level of Smad7 was increased. These effects suggested that osthole could inhibit collagen I and III expression and reduce their ratio via the TGF-β/Smad signaling pathway in TGF-β1 overexpressed CFs. These effects of osthole may play beneficial roles in the prevention and treatment of myocardial fibrosis.
