Chemical profiles and metabolite study of raw and processed Polygoni Multiflori Radix in rats by UPLC-LTQ-Orbitrap MS spectrometry.
10.1016/S1875-5364(18)30070-0
- Author:
Juan HUANG
1
;
Ju-Ping ZHANG
1
;
Jun-Qi BAI
1
;
Mei-Jin WEI
1
;
Jing ZHANG
1
;
Zhi-Hai HUANG
1
;
Guang-Hang QU
2
;
Wen XU
3
;
Xiao-Hui QIU
4
Author Information
1. The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
2. Shanghai Huayu Pharmaceutical Limited Company, Shanghai 200000, China.
3. The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, China. Electronic address: freexuwen@163.com.
4. The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, China. Electronic address: gxhqxh@medmail.com.cn.
- Publication Type:Journal Article
- Keywords:
Chemical profiles;
In vivo metabolite study;
Polygoni Multiflori Radix;
Processing
- MeSH:
Animals;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
metabolism;
Male;
Plant Preparations;
chemistry;
isolation & purification;
metabolism;
Plant Roots;
chemistry;
Polygonum;
chemistry;
Rats;
Rats, Sprague-Dawley;
Spectrum Analysis
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(5):375-400
- CountryChina
- Language:English
-
Abstract:
The raw and processed roots of Plygonum multiflorum Thunb (PM) are used to treat different diseases in clinical practice. In order to clarify the influence of processing, a comparative study of chemical substance analysis was carried out. As the xenobiotics with a high enough exposure in target organs being considered as the potential effective or toxicity components, an in vivo study was also implemented to characterize the constitutes and metabolites, and meanwhile, the factor of compatibility with black bean were also considered. As a result, a total of 148 compounds were detected in PM extracts and more than 40 compounds were only detected in the processed products, which were probably new components produced during the steaming process. In in vivo study, 7 prototype components and 66 metabolites were detected or tentatively identified, 24 of which were reported for the first time. Our results indicated that processing greatly changed the chemical composition of PM and influenced the disposition of the compounds in vivo. To the best of our knowledge, this was the first global comparative study of raw and processed PM. These results expanded our knowledge about the influence of processing of PM and provided the essential data for further efficacy or toxicity studies.
