Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways.
10.1016/S1875-5364(18)30083-9
- Author:
Qiang ZENG
1
,
2
;
Chun-Hay KO
1
,
3
,
4
;
Wing-Sum SIU
1
,
2
;
Kai-Kai LI
1
,
2
;
Chun-Wai WONG
1
,
2
;
Xiao-Qiang HAN
1
,
3
,
4
;
Liu YANG
5
;
Clara Bik-San LAU
1
,
3
,
4
;
Jiang-Miao HU
6
;
Ping-Chung LEUNG
1
,
3
,
7
Author Information
1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China.
3. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China
4. Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China.
5. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
6. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: hujiangmiao@mail.kib.ac.cn.
7. Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China. Electronic address: pingcleung@cuhk.edu.hk.
- Publication Type:Journal Article
- Keywords:
Anti-inflammation;
Dendrobium species;
MAPK pathways;
Macrophage
- MeSH:
Animals;
Anti-Inflammatory Agents;
pharmacology;
Cyclooxygenase 2;
genetics;
Cytokines;
metabolism;
Dendrobium;
chemistry;
Gene Expression Regulation, Enzymologic;
drug effects;
Inflammation;
chemically induced;
drug therapy;
Lipopolysaccharides;
Macrophages;
drug effects;
enzymology;
Mice;
Mitogen-Activated Protein Kinases;
antagonists & inhibitors;
genetics;
metabolism;
Nitric Oxide;
analysis;
Nitric Oxide Synthase Type II;
genetics;
Phosphorylation;
drug effects;
Plant Extracts;
pharmacology;
RAW 264.7 Cells;
Signal Transduction;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(7):481-489
- CountryChina
- Language:English
-
Abstract:
Dendrobii Caulis (DC), named 'Shihu' in Chinese, is a precious herb in traditional Chinese medicine. It is widely used to nourish stomach, enhance body fluid production, tonify "Yin" and reduce heat. More than thirty Dendrobium species are used as folk medicine. Some compounds from DC exhibit inhibitory effects on macrophage inflammation. In the present study, we compared the anti-inflammatory effects among eight Dendrobium species. The results provided evidences to support Dendrobium as folk medicine, which exerted its medicinal function partially by its inhibitory effects on inflammation. To investigate the anti-inflammatory effect of Dendrobium species, mouse macrophage cell line RAW264.7 was activated by lipopolysaccharide. The nitric oxide (NO) level was measured using Griess reagent while the pro-inflammatory cytokines were tested by ELISA. The protein expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) phosphorylation were evaluated by Western blotting analysis. Among the eight Dendrobium species, both water extracts of D. thyrsiflorum B.S.Williams (DTW) and D. chrysotoxum Lindl (DCHW) showed most significant inhibitory effects on NO production in a concentration-dependent manner. DTW also significantly reduced TNF-α, MCP-1, and IL-6 production. Further investigations showed that DTW suppressed iNOS and COX-2 expression as well as ERK and JNK phosphorylation, suggesting that the inhibitory effects of DTW on LPS-induced macrophage inflammation was through the suppression of MAPK pathways. In conclusion, D. thyrsiflorum B.S.Williams was demonstrated to have potential to be used as alternative or adjuvant therapy for inflammation.
