Moutan Cortex Radicis inhibits the nigrostriatal damage in a 6-OHDA-induced Parkinson's disease model.
10.1016/S1875-5364(18)30084-0
- Author:
Yeong-Gon CHOI
1
,
2
;
Yeon-Mi HONG
3
,
4
;
Li-Hua KIM
3
,
4
;
Sujung YEO
3
,
5
;
Sabina LIM
3
,
6
Author Information
1. Laboratory of Neurodegenerative Diseases, Ilsong Institute of Life Science, Hallym University, Anyang 14066, Republic of Korea
2. Research Group of Pain and Neuroscience, WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyung Hee University, Seoul 02447, Republic of Korea.
3. Research Group of Pain and Neuroscience, WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyung Hee University, Seoul 02447, Republic of Korea
4. Department of Applied Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
5. Department of Meridian and Acupoint, College of Korean Medicine, Sang Ji University, Wonju 26339, Republic of Korea.
6. Department of Applied Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea. Electronic address: lims@khu.ac.kr.
- Publication Type:Journal Article
- Keywords:
6-Hydroxydopamine;
Anti-inflammatory agent;
Neuroblastoma B65 cells;
Nitric oxide;
nNOS
- MeSH:
Animals;
Anti-Inflammatory Agents;
pharmacology;
therapeutic use;
Antiparkinson Agents;
pharmacology;
therapeutic use;
Cell Death;
drug effects;
Cell Line;
Disease Models, Animal;
Drugs, Chinese Herbal;
chemistry;
Neurons;
pathology;
Nitric Oxide;
analysis;
Nitric Oxide Synthase Type I;
biosynthesis;
Oxidopamine;
toxicity;
Paeonia;
chemistry;
Parkinsonian Disorders;
chemically induced;
drug therapy;
Phytotherapy;
Plant Extracts;
pharmacology;
therapeutic use;
Plants, Medicinal;
Rats;
Rats, Sprague-Dawley;
Substantia Nigra;
drug effects;
enzymology;
Tyrosine 3-Monooxygenase;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(7):490-498
- CountryChina
- Language:English
-
Abstract:
The traditionally used oriental herbal medicine Moutan Cortex Radicis [MCR; Paeonia Suffruticosa Andrews (Paeoniaceae)] exerts anti-inflammatory, anti-spasmodic, and analgesic effects. In the present study, we investigated the therapeutic effects of differently fractioned MCR extracts in a 6-hydroxydopamine (OHDA)-induced Parkinson's disease model and neuro-blastoma B65 cells. Ethanol-extracted MCR was fractionated by n-hexane, butanol, and distilled water. Adult Sprague-Dawley rats were treated first with 20 μg of 6-OHDA, followed by three MCR extract fractions (100 or 200 mg·kg) for 14 consecutive days. In the behavioral rotation experiment, the MCR extract-treated groups showed significantly decreased number of net turns compared with the 6-OHDA control group. The three fractions also significantly inhibited the reduction in tyrosine hydroxylase-positive cells in the substantia nigra pars compacta following 6-OHDA neurotoxicity. Western blotting analysis revealed significantly reduced tyrosine hydroxylase expression in the substantia nigra pars compacta in the 6-OHDA-treated group, which was significantly inhibited by the n-hexane or distilled water fractions of MCR. B65 cells were exposed to the extract fractions for 24 h prior to addition of 6-OHDA for 30 min; treatment with n-hexane or distilled water fractions of MCR reduced apoptotic cell death induced by 6-OHDA neurotoxicity and inhibited nitric oxide production and neuronal nitric oxide synthase expression. These results showed that n-hexane- and distilled water-fractioned MCR extracts inhibited 6-OHDA-induced neurotoxicity by suppressing nitric oxide production and neuronal nitric oxide synthase activity, suggesting that MCR extracts could serve as a novel candidate treatment for the patients with Parkinson's disease.
