1-Methoxycarbony-β-carboline from Picrasma quassioides exerts anti-angiogenic properties in HUVECs in vitro and zebrafish embryos in vivo.
10.1016/S1875-5364(18)30097-9
- Author:
Qing-Hua LIN
1
;
Wei QU
1
;
Jian XU
1
;
Feng FENG
2
,
3
;
Ming-Fang HE
4
Author Information
1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 211198, China.
2. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 211198, China
3. Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing 211198, China. Electronic address: fengfeng@cpu.edu.cn.
4. Institute of Translational Medicine, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China. Electronic address: mingfanghe@njtech.edu.cn.
- Publication Type:Journal Article
- Keywords:
1-Methoxycarbony-β-carboline;
Angiogenesis inhibitor;
Anti-angiogenic index;
HUVEC;
Zebrafish
- MeSH:
Angiogenesis Inhibitors;
chemistry;
pharmacology;
Animals;
Carbolines;
chemistry;
pharmacology;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Epidermal Growth Factor;
genetics;
metabolism;
Fibroblast Growth Factors;
genetics;
metabolism;
Human Umbilical Vein Endothelial Cells;
cytology;
drug effects;
metabolism;
Humans;
Insulin-Like Growth Factor I;
genetics;
metabolism;
Neovascularization, Physiologic;
drug effects;
Picrasma;
chemistry;
Plant Extracts;
chemistry;
pharmacology;
Receptor, TIE-2;
genetics;
metabolism;
Zebrafish;
embryology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(8):599-609
- CountryChina
- Language:English
-
Abstract:
Angiogenesis is a crucial process in the development of inflammatory diseases, including cancer, psoriasis and rheumatoid arthritis. Recently, several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model, and the results indicated that 1-methoxycarbony-β-carboline (MCC) could effectively inhibit blood vessel formation. In this study, we further confirmed that MCC can inhibit, in a concentration-dependent manner, the viability, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo. In the zebrafish xenograft assay, MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells. The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins, including ANG, EGF, bFGF, GRO, IGF-1, PLG and MMP-1. In addition, the expression of two key membrane receptor proteins in angiogenesis, TIE-2 and uPAR, were also down-regulated after MCC treatment. Taken together, these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
