Anti-parasitic effects of water-soluble alkaloid fractions from ethanolic extracts of Sophora moorcroftiana seeds in Caenorhabditis elegans.
10.1016/S1875-5364(18)30106-7
- Author:
Yan-Ping LUO
1
,
2
;
Yuan ZHANG
3
;
Hui-Min ZHANG
3
;
Hong ZHANG
3
;
Lin ZHANG
3
;
Hong-Juan YU
3
;
Ming-Qiang CAO
3
;
Yan-Bin SHI
4
;
De-Juan ZHI
4
;
Xing-Ming MA
1
,
5
;
Kai-Zhong DONG
6
Author Information
1. Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
2. Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, China.
3. Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
4. School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
5. Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, China. Electronic address: maxm@lzu.edu.cn.
6. Department of Microbiology, Medical College, Northwest Minzu University, Lanzhou 730000, China. Electronic address: dkz@xbmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Alkaloids;
Anthelmintics;
Caenorhabditis elegans;
Protoscoleces;
Sophora moorcroftiana
- MeSH:
Animals;
Anthelmintics;
administration & dosage;
isolation & purification;
Caenorhabditis elegans;
drug effects;
physiology;
China;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
Echinococcosis;
drug therapy;
parasitology;
Echinococcus granulosus;
drug effects;
physiology;
Humans;
Seeds;
chemistry;
Sophora;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(9):665-673
- CountryChina
- Language:English
-
Abstract:
Parasite infections of humans and animals remain a major global health problem, with limited choice of drugs being available to the treatment of parasitosis in the clinic. Sophora moorcroftiana (S. moorcroftiana) is a shrub that grows in Tibet Plateau of China. Decoction of the seeds has been used as a traditional Tibetan medicine to treat parasitosis for years. But the anti-parasitic effects of water-soluble fractions in the seeds need further investigation. In the present study, the water-soluble alkaloid fractions (E2) were obtained from S. moorcroftiana seeds by refluxing extraction with 60% ethanol and low polarity fraction (E2-a) and high polarity fraction (E2-b) were subsequently isolated from E2 using column chromatography. As a parasite model, Caenorhabditis elegans (C. elegans) were treated with different fractions and their survivals were recorded. The results showed that that E2-a induced a lower survival rate in C. elegans than E2-b and E2. The protoscoleces of Echinococcus granulosus (E. granulosus) were cultured in the presence of E2-a. Compared with E2-b and E2, protoscoleces exhibited decreased survival rate following E2-a treatment. Furtherly, the effects of E2-a on the behavior, brood size, and lifespan of the worms were investigated. Body bend frequencies of the worms treated with the high concentration of E2-a were reduced by two-thirds compared with the control group (P < 0.01). Compared with non-E2-a-treated group, exposure of nematodes to E2-a led to a decrease in head thrashes and pharyngeal pumps frequency (P < 0.01). E2-a treatment resulted in a significantly lower brood size (P < 0.01). Additional E2-a treatment induced a significantly shortened lifespan, compared with the control (P < 0.05). These findings indicated that water-soluble fraction E2-a from S. moorcroftiana seeds was a potential helminthic agent.
