Evaluation of neuroactive effects of ethanol extract of Schisandra chinensis, Schisandrin, and Schisandrin B and determination of underlying mechanisms by zebrafish behavioral profiling.
10.1016/S1875-5364(18)30133-X
- Author:
Jia-Wei WANG
1
;
Feng-Yin LIANG
2
;
Xiang-Shuo OUYANG
1
;
Pei-Bo LI
1
;
Zhong PEI
3
;
Wei-Wei SU
4
Author Information
1. Guangdong Engineering and Technology Research Centre for Quality and Efficacy Re-evaluation of Post-marketed TCM, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
2. Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
3. Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. Electronic address: peizhong@mail.sysu.edu.cn.
4. Guangdong Engineering and Technology Research Centre for Quality and Efficacy Re-evaluation of Post-marketed TCM, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: lssswwhk@gmail.com.
- Publication Type:Journal Article
- Keywords:
Behavior profiling;
Neurotransmitter;
Schisandra chinensis;
Traditional Chinese medicine;
Zebrafish
- MeSH:
Animals;
Behavior, Animal;
drug effects;
Caenorhabditis elegans;
Central Nervous System Agents;
chemistry;
isolation & purification;
pharmacology;
Cyclooctanes;
analysis;
isolation & purification;
pharmacology;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Lignans;
analysis;
isolation & purification;
pharmacology;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology;
Polycyclic Compounds;
analysis;
isolation & purification;
pharmacology;
Schisandra;
chemistry;
Zebrafish;
physiology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(12):916-925
- CountryChina
- Language:English
-
Abstract:
Schisandra chinensis, a traditional Chinese medicine (TCM), has been used to treat sleep disorders. Zebrafish sleep/wake behavioral profiling provides a high-throughput platform to screen chemicals, but has never been used to study extracts and components from TCM. In the present study, the ethanol extract of Schisandra chinensis and its two main lignin components, schisandrin and schisandrin B, were studied in zebrafish. We found that the ethanol extract had bidirectional improvement in rest and activity in zebrafish. Schisandrin and schisandrin B were both sedative and active components. We predicted that schisandrin was related to serotonin pathway and the enthanol extract of Schisandra chinensis was related to seoronin and domapine pathways using a database of zebrafish behaviors. These predictions were confirmed in experiments using Caenorhabditis elegans. In conclusion, zebrafish behavior profiling could be used as a high-throughput platform to screen neuroactive effects and predict molecular pathways of extracts and components from TCM.
