Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo.
10.1016/S1875-5364(13)60094-1
- Author:
Guang-Ming YANG
1
;
Ru YAN
2
;
Zhao-Xian WANG
3
;
Fang-Fang ZHANG
1
;
Yang PAN
4
,
5
;
Bao-Chang CAI
6
Author Information
1. Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing & Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. University of Macau, Macau, China.
3. School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
4. Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing & Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
5. Laboratory of Medical Fungi and Phyto-Biotech, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: y.pan2006@163.com.
6. Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing & Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: bccai@126.com.
- Publication Type:Journal Article
- Keywords:
Antitumor;
Apoptosis;
Murine hepatoma22;
Oxytropis falcata;
Proliferation;
SMMC-7721
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
administration & dosage;
Apoptosis;
drug effects;
Carcinoma, Hepatocellular;
drug therapy;
physiopathology;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
administration & dosage;
Growth Inhibitors;
administration & dosage;
Humans;
Liver Neoplasms;
drug therapy;
physiopathology;
Male;
Mice;
Mice, Inbred ICR;
Oxytropis;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(5):519-524
- CountryChina
- Language:English
-
Abstract:
AIMS:To investigate the antitumor effects of extracts from Oxytropis falcata on human hepatocellular carcinoma SMMC-7721 cells in vitro and in transplanted murine H22 tumors in vivo.
METHODS:Cell proliferation, cell cycle distribution and apoptosis in SMMC-7721 cells were determined and tumor growth inhibition in H22 tumors was investigated. Cell cycle distribution was analyzed by flow cytometry with propidium iodide (PI) and Annexin V-FITC/ PI double staining.
RESULTS:MTT assay revealed that essential oil and flavonoids of O. falcata (named EOOF and FOF) inhibited proliferation of SMMC-7721 cells in a dose-dependent manner. The IC50 value of EOOF and FOF were 0.115 and 0.097 mg·mL(-1), respectively. Cell cycle was arrested at G(1) phase, and induction of apoptosis occurred in SMMC-7721 cells when subjected to FOF. Growth inhibition in H22 solid tumors transplanted mice was significantly pronounced after being treated with FOF, and the inhibition ratio were 56.1% and 70.8% at the concentration of 30 and 60 mg·kg(-1).
CONCLUSION:The results suggest that FOF promotes apoptosis in SMMC-7721 cells and inhibits H22 tumor growth, resulting in a potential antitumor effect on hepatic cancer.
