LC-MS/MS method for the determination of a new puerarin derivative and its application in pharmacokinetic studies in rats.
10.1016/S1875-5364(13)60102-8
- Author:
An-Chang LIU
1
;
Li-Xia ZHAO
1
;
Jie XING
2
;
Jian GAO
2
;
Hong-Xiang LOU
3
Author Information
1. Qilu Hospital of Shandong University, Jinan 250012, China.
2. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
3. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: louhongxiang@sdu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Dehydrated puerarin;
Genistein;
LC-MS/MS;
Pharmacokinetics
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
methods;
Drug Stability;
Drugs, Chinese Herbal;
administration & dosage;
analysis;
metabolism;
pharmacokinetics;
Female;
Isoflavones;
administration & dosage;
blood;
metabolism;
pharmacokinetics;
Male;
Pueraria;
chemistry;
Rats;
Rats, Wistar;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(5):566-571
- CountryChina
- Language:English
-
Abstract:
AIM:To establish a sensitive and rapid liquid chromatographic-tandem mass spectrometry (LC-MS/MS) method for the quantitative analysis of dehydrated puerarin in rat plasma, and its application for pharmacokinetic studies.
METHODS:A plasma sample was pretreated by one-step protein precipitation by the addition of five volumes of methanol. The chromatographic separation was achieved on a Zorbax SB-C18 column (4.6 mm × 150 mm I.D. 5.0 μm, Agilent, USA) at 40 °C at a flow rate of 0.6 mL·min(-1) by an isocratic elution consisting of 10 mmol·L(-1) ammonium acetate in methanol and water containing 0.1% formic acid in a ratio of 20 : 80 (V/V). Detection was performed on a triple quadrupole mass spectrometer in multiple-reaction monitoring (MRM) mode. An atmospheric pressure chemical ionization (APCI) interface in positive ionization mode was used by monitoring the transitions from m/z 399.1→281.0 (dehydrated puerarin) and m/z 271.0→215.0 (internal standard, IS).
RESULTS:Calibration curves were linear in the concentration range from 1.50 to 5400 ng·mL(-1), and the lower limit of quantification (LLOQ) was 1.50 ng·mL(-1) in rat plasma. The accuracy and precision values, which were calculated from three different sets of quality control samples analyzed in sextuplicate on three different days, ranged from 95.73% to 103.18%, and from 4.33% to 7.86%, respectively.
CONCLUSION:The method was successfully applied to assess the pharmacokinetics of dehydrated puerarin after oral administration in rats.
