Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies.
10.1016/S1875-5364(13)60103-X
- Author:
Shi-Jia LIU
1
,
2
;
Pei-Dong CHEN
3
;
Guo-Liang DAI
3
;
Wen-Zheng JU
4
;
Li-Yan XIE
3
;
Jie XU
3
;
Ling ZHOU
3
;
An-Wei DING
3
;
Bo-Yang YU
5
Author Information
1. Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China
2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
3. Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
4. Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China. Electronic address: snow8185@yahoo.com.cn.
5. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.
- Publication Type:Journal Article
- Keywords:
Electrospray ionization;
Isorhamnetin-3-O-neohesperidoside;
LC-MS/MS;
Pharmacokinetics;
Rat plasma
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
administration & dosage;
analysis;
pharmacokinetics;
Flavonols;
administration & dosage;
blood;
pharmacokinetics;
Pollen;
chemistry;
Rats;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods;
Typhaceae;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(5):572-576
- CountryChina
- Language:English
-
Abstract:
AIM:To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.
METHODS:Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 : 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.
RESULTS:The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).
CONCLUSION:The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.
