DT-13, a saponin of dwarf lilyturf tuber, exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells.
10.1016/S1875-5364(14)60005-4
- Author:
Zhao REN-PING
1
;
Lin SEN-SEN
2
;
Sheng-Tao YUAN
3
,
4
;
Bo-Yang YU
5
;
Xian-Shu BAI
6
;
Li SUN
7
;
Lu-Yong ZHANG
8
,
9
Author Information
1. Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
2. Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing 210009, China.
3. Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing 210009, China
4. Department of Complex Prescription of Traditional Chinese Medicine, School of Chinese Material Medicine, China Pharmaceutical University, Nanjing 210009, China.
5. Department of Complex Prescription of Traditional Chinese Medicine, School of Chinese Material Medicine, China Pharmaceutical University, Nanjing 210009, China.
6. Department of Molecular Physiology, University of Saarland, 66421 Homburg, Germany.
7. Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: cpusunli@126.com.
8. Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China
9. Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China. Electronic address: drugscreen@163.com.
- Publication Type:Journal Article
- Keywords:
Anticancer activity;
CCR5;
DT-13;
HIF-1α;
Ophiopogon japonicus;
R285;
Saponin;
VEGF
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
administration & dosage;
Breast Neoplasms;
drug therapy;
genetics;
metabolism;
physiopathology;
CCR5 Receptor Antagonists;
Cell Adhesion;
drug effects;
Cell Line, Tumor;
Cell Movement;
drug effects;
Drugs, Chinese Herbal;
administration & dosage;
Female;
Humans;
Liriope Plant;
chemistry;
Mice;
Mice, Nude;
Plant Tubers;
chemistry;
Receptors, CCR5;
genetics;
metabolism;
Saponins;
administration & dosage;
Vascular Endothelial Growth Factor A;
antagonists & inhibitors;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(1):24-29
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action.
METHODS:MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogon japonicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor (VEGF), C-C chemokine receptor type 5 (CCR5) and hypoxia-inducible factor 1α (HIF-1α) were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay.
RESULTS:At 0.01 to 1 μmol·L(-1), DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1α. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo.
CONCLUSION:DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-1α expression.
