Comparison of anti-bacterial activity of three types of di-O-caffeoylquinic acids in Lonicera japonica flowers based on microcalorimetry.
10.1016/S1875-5364(14)60017-0
- Author:
Jin HAN
1
;
Qing-Yuan LV
1
;
Shi-Ying JIN
1
;
Tian-Tian ZHANG
1
;
Shi-Xiao JIN
1
;
Xian-Yi LI
2
;
Hai-Long YUAN
3
Author Information
1. 302 Military Hospital of China, Beijing 100039, China.
2. Institute for Drug and Instrument Control, Health Department, GLD of PLA, Beijing 100071, China. Electronic address: lxyynz@126.com.
3. 302 Military Hospital of China, Beijing 100039, China. Electronic address: yhlpharm@126.com.
- Publication Type:Journal Article
- Keywords:
Antibacterial activity;
Bacillus shigae;
Lonicera japonica;
Microcalorimetry;
di-O-caffeoylquinic acids
- MeSH:
Anti-Bacterial Agents;
pharmacology;
Bacillus;
drug effects;
growth & development;
Chlorogenic Acid;
analogs & derivatives;
chemistry;
pharmacology;
Drugs, Chinese Herbal;
pharmacology;
Flowers;
chemistry;
Inhibitory Concentration 50;
Lonicera;
chemistry;
Monosaccharides;
chemistry;
pharmacology;
Quinic Acid;
analogs & derivatives;
chemistry;
pharmacology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(2):108-113
- CountryChina
- Language:English
-
Abstract:
The anti-bacterial activities of three types of di-O-caffeoylquinic acids (diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine (TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid (3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid (3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid (4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power-time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs (IC50) were obtained by quantitative analysis. Based on the quantity-activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.
