Cytotoxicity and anti-inflammatory effects of root bark extracts of Acanthopanax henryi.
10.1016/S1875-5364(14)60019-4
- Author:
Jong-Hwan KIM
1
;
Xiang-Qian LIU
2
;
Ling DAI
3
;
Chang-Soo YOOK
4
;
Kyung-Tae LEE
4
Author Information
1. Herbal Medicine Research Division, Korea Food & Drug Administration, Seoul 363-700, Korea.
2. Hunan Key Laboratory of Traditional Chinese Medicine modernization, School of Pharmacy, Hunan University of Chinese Medicine, Hunan Changsha 410208, China. Electronic address: lxq0001cn@163.com.
3. Hunan Key Laboratory of Traditional Chinese Medicine modernization, School of Pharmacy, Hunan University of Chinese Medicine, Hunan Changsha 410208, China.
4. College of Pharmacy, Kyung-Hee University, Seoul 130-701, Korea.
- Publication Type:Journal Article
- Keywords:
Acanthopanax henryi (Oliv.) Harms;
Anti-inflammatory activity;
Cytotoxic activity;
Root bark extract
- MeSH:
Animals;
Anti-Inflammatory Agents;
pharmacology;
therapeutic use;
Dinoprostone;
metabolism;
Dose-Response Relationship, Drug;
Eleutherococcus;
Inflammation;
chemically induced;
drug therapy;
metabolism;
Interleukin-1beta;
metabolism;
Interleukin-6;
metabolism;
Lipopolysaccharides;
Macrophages;
drug effects;
metabolism;
Mice;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
metabolism;
Phytotherapy;
Plant Bark;
Plant Extracts;
pharmacology;
therapeutic use;
Plant Roots
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(2):121-125
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi.
METHOD:The hot methanol extract of the root bark of A. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibition on lipopolysaccharide (LPS)-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages.
RESULTS:The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase (iNOS) at the protein levels in a concentration-dependent manner.
CONCLUSION:The 80% MeOH fraction of A. henryi root bark has significant anti-inflammatory activity. This provides a pharmacological basis for clinical application for the treatment of inflammation.
