Histological and ultrastructural studies on the toxic effect of pan masala and its amelioration by Elettaria cardamomum.
10.1016/S1875-5364(14)60033-9
- Author:
Sweety KUMARI
1
;
Abhijit DUTTA
2
Author Information
1. University Department of Zoology, Ranchi University, Ranchi-834008, India. Electronic address: sweety25j@gmail.com.
2. University Department of Zoology, Ranchi University, Ranchi-834008, India.
- Publication Type:Journal Article
- Keywords:
Elettaria cardamomum;
Histology;
Pan masala;
Testes;
Ultrastructure
- MeSH:
Animals;
Areca;
adverse effects;
Elettaria;
Male;
Mice;
Phytotherapy;
Plant Extracts;
pharmacology;
therapeutic use;
Spermatogenesis;
drug effects;
Spermatozoa;
drug effects;
Testicular Diseases;
chemically induced;
drug therapy;
pathology;
Testis;
drug effects;
pathology;
ultrastructure;
Tobacco;
adverse effects;
Tobacco, Smokeless;
adverse effects;
Vacuoles;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(3):199-203
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the histological and ultrastructural changes observed in pan masala intoxicated mammalian testes under the effect of cardamom.
METHODS:Male Swiss mice were given pan masala orally at a dose of 2% of the feed and cardamom at a dose of 0.2% of the feed. They were divided into three groups, control (Group I), pan masala-treated (Group II), and a combination of pan masala and cardamom-treated group (Group III). Histologically, the testes of Group II mice displayed degeneration of tubular epithelium, disruption of spermatogenesis, and a marked reduction in germ cells.
RESULTS:When cardamom was given, damage was less with fewer distorted cells and also improvement with normal tubules and spermatid differentiation in Group III. Ultrastructurally, pan masala-treated testes showed cytoplasmic vacuolation, shrinkage and pyknotic nuclei of spermatogonia, and abnormal acrosomal granules.
CONCLUSION:When cardamom was given, the amelioration process was more evident showing a comparable morphology with control.
