A new cytochalasin from endophytic Phomopsis sp. IFB-E060.
10.1016/S1875-5364(14)60080-7
- Author:
Li SHEN
1
;
Qian LUO
2
,
3
;
Zhi-Ping SHEN
1
;
Ling-Yu LI
1
;
Xiao-Jun ZHANG
1
;
Zhong-Qi WEI
1
;
Yi FU
1
;
Yong-Chun SONG
4
;
Ren-Xiang TAN
5
,
6
Author Information
1. Medical College, Yangzhou University, Yangzhou 225009, China.
2. Medical College, Yangzhou University, Yangzhou 225009, China
3. State Key Laboratory of Element Organic Chemistry, Nankai University, Tianjin 300071, China.
4. Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
5. State Key Laboratory of Element Organic Chemistry, Nankai University, Tianjin 300071, China
6. Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China. Electronic address: rxtan@nju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cytochalasin;
Endophyte;
Phomopsis sp.;
Sphaeropsidaceae;
Vatica mangachapoi
- MeSH:
Ascomycota;
chemistry;
isolation & purification;
Cell Line, Tumor;
Cell Survival;
drug effects;
Cytochalasins;
chemistry;
isolation & purification;
toxicity;
Endophytes;
chemistry;
isolation & purification;
Humans;
Magnoliopsida;
microbiology;
Molecular Structure;
Plant Bark;
microbiology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(7):512-516
- CountryChina
- Language:English
-
Abstract:
AIM:To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi.
METHOD:Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses (UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721.
RESULTS:Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J (1), cytochalasin H (2), (22E, 24S)-cerevisterol (3), ergosterol (4), and nicotinic acid (5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL(-1) and 2 had an IC50 value of 15.0 μg·mL(-1), while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL(-1).
CONCLUSION:18-Methoxy cytochalasin J (1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.
