Screening and identification of multiple components in Tanreqing injection using RP-HPLC combined with DAD and ESI-TOF/MS.
10.1016/S1875-5364(14)60083-2
- Author:
Shao-Yong LIU
1
;
Dong-Sheng XUE
1
;
Jian-Chao PAN
1
;
Wen-Ming ZHANG
1
;
Wen-Long LI
2
;
Hai-Bin QU
3
Author Information
1. Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China.
2. Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China. Electronic address: wshlwl@zju.edu.cn.
3. Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- Keywords:
DAD;
ESI-TOF/MS;
Screening and identification;
Tanreqing injection
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Molecular Structure;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(7):535-541
- CountryChina
- Language:English
-
Abstract:
A liquid chromatography coupled with diode array detector (DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) method was developed for the screening and identification of the multiple components in Tanreqing injection, a well-known Chinese medicine injection in China. By combining the DAD spectrum and the accurate mass measurement of ESI-TOF/MS, twelve components in Tanreqing injection were identified. This study contributes to clarifying the nature of Tanreqing injection, and provides an effective and reliable process for the comprehensive and systematic characterization of complex traditional Chinese medicine preparations.
