Buspirone along with melatonin attenuates oxidative damage and anxiety-like behavior in a mouse model of immobilization stress.
10.1016/S1875-5364(14)60089-3
- Author:
Anil KUMAR
1
;
Gurleen KAUR
2
;
Puneet RINWA
2
Author Information
1. Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh. PIN-160014. Electronic address: kumaruips@yahoo.com.
2. Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh. PIN-160014.
- Publication Type:Journal Article
- Keywords:
Anxiety;
Buspirone;
Immobilization;
Melatonin;
Oxidative stress
- MeSH:
Animals;
Anti-Anxiety Agents;
pharmacology;
therapeutic use;
Antioxidants;
pharmacology;
therapeutic use;
Anxiety;
drug therapy;
Behavior, Animal;
drug effects;
Buspirone;
pharmacology;
therapeutic use;
Immobilization;
psychology;
Male;
Melatonin;
pharmacology;
therapeutic use;
Mice, Inbred Strains;
Oxidative Stress;
drug effects;
Stress, Psychological;
drug therapy
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(8):582-589
- CountryChina
- Language:English
-
Abstract:
AIM:Stress is recognized to precipitate anxiety and related psychological problems characterized by a wide range of biochemical and behavioral changes. The present study was carried out to investigate the protective effects of melatonin and buspirone, and their combination, against six hours immobilization stress-induced, anxiety-like behavioral and oxidative damage in mice.
METHOD:Male Laca mice were pre-treated with melatonin (2.5, 5 mg·kg(-1)), buspirone (5, 10 mg·kg(-1)), and their combination for consecutive five days. On the 6(th) day, animals were immobilized for six hours, and thereafter various behavioral tests were performed followed by biochemical tests.
RESULTS:Immobilization stress significantly impaired body weight, locomotor activity, and caused anxiety-like behavior, along with increased oxidative damage. Pretreatment with melatonin and buspirone significantly improved the loss in body weight and locomotor activity, attenuated anxiety-like behavior (in both the mirror chamber and plus maze performance tasks), further restored the levels of brain total proteins, and caused antioxidant-like effects, as evidenced by reduced lipid peroxidation, nitrite concentration, and restoration of reduced glutathione and catalase activity, as compared to control animals. In addition, combination of melatonin (2.5, 5 mg·kg(-1)) with buspirone (5 mg·kg(-1)) significantly potentiated their protective effects, as compared to their effects individually.
CONCLUSION:The present study suggests that melatonin potentiates the beneficial effect of buspirone against immobilization stress-induced, anxiety-like behavioral and oxidative damage in mice possibly by involving a serotonergic mechanism.
