Quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside metabolites in the rat using UPLC-Q-TOF/MS.
10.1016/S1875-5364(14)60109-6
- Author:
Xin YAO
1
,
2
;
Gui-Sheng ZHOU
3
;
Yu-Ping TANG
4
;
Er-Xin SHANG
3
;
Jian-Ming GUO
3
;
Da-Wei QIAN
3
;
Jin-Ao DUAN
3
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
3. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
4. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: yupingtang@njutcm.edu.cn.
- Publication Type:Journal Article
- Keywords:
Metabolite identification;
Quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside;
UPLC-Q-TOF/MS
- MeSH:
Administration, Intravenous;
Animals;
Chromatography, High Pressure Liquid;
Ginkgo biloba;
chemistry;
Male;
Mass Spectrometry;
methods;
Plant Extracts;
metabolism;
Quercetin;
analogs & derivatives;
metabolism;
Rats, Sprague-Dawley
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(9):705-711
- CountryChina
- Language:English
-
Abstract:
Ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the Metabolynx™ software, combined with mass defect filtering, were applied to identity the metabolites of quercetin-3-O-β-D-gluco-pyranosyl-(4→1)-α-L-rhamnoside (QGR) in rats after intravenous administration. MS(E) was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the rapid structural characterization of eight metabolites in rat plasma, urine and bile. The results indicated that methylation and glucuronidation were the major metabolic pathways of QGR in vivo. The present study provided important information about the metabolism of QGR which will be useful for fully understanding the mechanism of action of this compound. Furthermore, this work demonstrated the potential of the UPLC-Q-TOF/MS approach using Metabolynx for rapid and automated research of the metabolites of natural products.
