Characterization of acid-and pepsin-soluble collagens from spines and skulls of skipjack tuna (Katsuwonus pelamis).
10.1016/S1875-5364(14)60110-2
- Author:
Di YU
1
;
Chang-Feng CHI
1
;
Bin WANG
2
;
Guo-Fang DING
3
;
Zhong-Rui LI
1
Author Information
1. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China.
2. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: wangbin4159@hotmail.com.
3. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: dinggf@zjou.edu.cn.
- Publication Type:Journal Article
- Keywords:
Acid soluble collagen (ASC);
Characterization;
Katsuwonus pelamis;
Pepsin soluble collagen (PSC);
Skipjack tuna;
Skull;
Spine
- MeSH:
Acids;
chemistry;
Amino Acids;
analysis;
Animals;
Collagen;
chemistry;
isolation & purification;
Collagen Type I;
chemistry;
isolation & purification;
Hydrogen-Ion Concentration;
Molecular Structure;
Molecular Weight;
Pepsin A;
chemistry;
Skull;
chemistry;
Sodium Chloride;
Solubility;
Spine;
chemistry;
Temperature;
Tuna
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(9):712-720
- CountryChina
- Language:English
-
Abstract:
Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the spine (ASC-SP and PSC-SP) and skull (ASC-SK and PSC-SK) of the skipjack tuna, Katsuwonus pelamis, were successfully isolated and characterized. The yields of ASC-SP, PSC-SP, ASC-SK and PSC-SK were (2.47 ± 0.39)%, (5.62 ± 0.82)%, (3.57 ± 0.40)%, and (6.71 ± 0.81)%, respectively, on the basis of dry weight. The four collagens contained Gly (330.2-339.1 residues/1 000 residues) as the major amino acid, and their imino acid contents were between 168.8 and 178.2 residues/1 000 residues. Amino acid composition, SDS-PAGE, and FTIR investigations confirmed that ASC-SP and ASC-SK were mainly composed of type I collagen, and had higher contents of high-molecular weight cross-links than those of PSC-SK and PSC-SP. The FTIR investigation also certified all the collagens had triple helical structure. The denaturation temperatures of ASC-SK, PSC-SK, ASC-SP, and PSC-SP were 17.8, 16.6, 17.6, and 16.5 °C, respectively. All isolated collagens were soluble at acidic pH (1-5) and lost their solubilities when the NaCl concentration was above 2% (W/V). The isolated collagens from the spines and skulls of skipjack tuna could serve as an alternative source of collagens for further application in food, cosmetic, biomedical, and pharmaceutical industries.
