Tetramethylpyrazine protects lymphocytes from radiation-induced apoptosis through nuclear factor-κB.
10.1016/S1875-5364(14)60112-6
- Author:
Xiao-Yan WANG
1
,
2
;
Zeng-Chun MA
3
;
Yu-Guang WANG
4
;
Hong-Ling TAN
4
;
Cheng-Rong XIAO
4
;
Qian-De LIANG
4
;
Xiang-Lin TANG
4
;
Yu CHENG
5
;
Yue GAO
6
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China
2. Chongqing Maternal and Child Health-Care Hospital, Chongqing 400016, China.
3. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: 13681121635@139.com.
4. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
5. Chongqing Maternal and Child Health-Care Hospital, Chongqing 400016, China.
6. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: gaoyue@bmi.ac.cn.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
NF-κB;
Radioprotection;
Tetramethylpyrazine
- MeSH:
Apoptosis;
drug effects;
radiation effects;
Cell Line;
Cell Survival;
drug effects;
radiation effects;
DNA Fragmentation;
drug effects;
radiation effects;
Drugs, Chinese Herbal;
pharmacology;
Humans;
Lymphocytes;
cytology;
drug effects;
radiation effects;
NF-kappa B;
genetics;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Pyrazines;
pharmacology;
Radiation-Protective Agents;
pharmacology;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(10):730-737
- CountryChina
- Language:English
-
Abstract:
AIM:Radiation induces an important apoptosis response in irradiated organs. The objective of this study was to investigate the radioprotective effect of tetramethylpyrazine (TMP) on irradiated lymphocytes and discover the possible mechanism of protection.
METHOD:Lymphocytes were pretreated for 12 h with TMP (25-200 μmol·L(-1)) and then exposed to 4 Gy radiation. Cell apoptosis and the signaling pathway were analyzed.
RESULTS:Irradiation increased cell death, DNA fragmentation, activated caspase activation and cytochrome c translocation, downregulated B-cell lymphoma 2 (Bcl-2) and up-regulated Bcl-2-associated X protein (Bax). Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit caspase-9 activation, and upregulate Bcl-2 and down-regulate Bax in 4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB (NF-κB) from cytosol into the nucleus.
CONCLUSION:The results suggest that TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis.
