Role of Ocimum sanctum leaf extract on dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
10.1016/S1875-5364(14)60118-7
- Author:
Yasir Hasan SIDDIQUE
1
;
Mohammad FAISAL
2
;
Falaq NAZ
3
;
Smita JYOTI
3
;
RAHUL
3
Author Information
1. Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India. Electronic address: yasir_hasansiddique@rediffmail.com.
2. Forest Entomology Division, Forest Research Institute, Dehradun 248006, UK, India.
3. Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
- Publication Type:Journal Article
- Keywords:
Climbing assay;
Drosophila model;
Lipid peroxidation;
Ocimum sanctum;
Parkinson's disease
- MeSH:
Animals;
Brain;
drug effects;
metabolism;
Dietary Supplements;
analysis;
Disease Models, Animal;
Drosophila;
drug effects;
genetics;
metabolism;
Humans;
Lipid Peroxidation;
drug effects;
Ocimum;
chemistry;
Oxidative Stress;
drug effects;
Parkinson Disease;
drug therapy;
metabolism;
Plant Extracts;
administration & dosage;
Plant Leaves;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(10):777-781
- CountryChina
- Language:English
-
Abstract:
AIM:To evaluate the effect of Ocimum sanctum leaf extract on the dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
METHOD:The effect of Ocimum sanctum leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αs) in the neurons. O. sanctum extract at final concentrations of 0.042 8 × 10(-4), 0.87 × 10(-4), and 1.85 × 10(-4) g·mL(-1) of diet were established and the flies were allowed to feed for 21 days. The climbing assay and lipid peroxidation were taken as parameters for the study.
RESULTS:The supplementation of O. sanctum extract showed a dose-dependent significant delay in the loss of climbing ability and reduction in oxidative stress in the brain of PD model flies.
CONCLUSION:The results of the present study showed that the O. sanctum extract is potent in reducing the PD symptoms in transgenic Drosophila model.
