Simultaneous quantitative determination of five alkaloids in Catharanthus roseus by HPLC-ESI-MS/MS.
10.1016/S1875-5364(14)60120-5
- Author:
Lin ZHANG
1
,
2
,
3
,
4
;
Qing-Hui GAI
1
,
2
,
3
,
4
;
Yuan-Gang ZU
1
,
2
,
3
,
5
;
Lei YANG
1
,
2
,
3
,
4
;
Yu-Liang MA
1
,
2
,
3
,
4
;
Yang LIU
1
,
2
,
3
,
4
Author Information
1. State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China
2. Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China
3. Key Laboratory of Forest Plant Ecology, Ministry of Education
4. Northeast Forestry University, Harbin, 150040, China.
5. Northeast Forestry University, Harbin, 150040, China. Electronic address: zuyuanggang@163.com.
- Publication Type:Journal Article
- Keywords:
Catharanthus roseus;
HPLC-ESI-MS/MS;
Monomeric and bisindole alkaloids
- MeSH:
Alkaloids;
chemistry;
Catharanthus;
chemistry;
China;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(10):786-793
- CountryChina
- Language:English
-
Abstract:
AIM:To establish a method to simultaneously determine the main five alkaloids of Catharanthus roseus for trace samples, a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) analysis method was developed.
METHOD:The five Catharanthus alkaloids, vinblastine, vincristine, vinleurosine, vindoline, and catharanthine were chromatographically separated on a C18 HPLC column. The mobile phase was methanol-15 nmol·L(-1) ammonium acetate containing 0.02% formic acid (65 : 35, V/V). The quantification of these alkaloids was based on the Multiple Reaction Monitoring (MRM) mode.
RESULTS:This method was validated, and the results achieved the aims of the study. The intra- and inter-day precision and accuracy of the five alkaloids were within 1.2%-11.5% (RSD%) and -10.9%-10.5% (RE%). The recovery rates of the five alkaloids of samples were from 79.9% to 91.5%. The five analytes were stable at room temperature for 2 h, at 4 °C for 12 h, and at -20 °C for two weeks. The developed method was applied successfully to determine the content of the five alkaloids in three plant parts of three batches of C. roseus with a minute amount collected from three regions of China.
CONCLUSION:The HPLC-ESI-MS/MS method can be used for the simultaneous determination of five important alkaloids in trace C. roseus samples.
