Tectorigenin inhibits the inflammation of LPS-induced acute lung injury in mice.
10.1016/S1875-5364(14)60126-6
- Author:
Chun-Hua MA
1
,
2
;
Ji-Ping LIU
3
;
Rong QU
4
;
Shi-Ping MA
5
Author Information
1. Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China
2. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
3. Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
4. Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China. Electronic address: qur2004@yahoo.com.cn.
5. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. Electronic address: spma@cpu.edu.com.
- Publication Type:Journal Article
- Keywords:
Inflammation;
LPS-induced acute lung injury;
Tectorigenin
- MeSH:
Acute Lung Injury;
chemically induced;
drug therapy;
pathology;
Animals;
Bronchoalveolar Lavage Fluid;
cytology;
Cell Count;
Female;
Inflammation;
drug therapy;
pathology;
Isoflavones;
therapeutic use;
Lipopolysaccharides;
Mice;
Mice, Inbred BALB C;
Peroxidase;
analysis;
Pulmonary Edema;
pathology;
Superoxide Dismutase;
analysis
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(11):841-846
- CountryChina
- Language:English
-
Abstract:
AIM:In a previous study, the anti-inflammatory effects of tectorigenin were disclosed. In this study, the anti-inflammatory effects of tectorigenin on acute lung injury using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model were investigated
METHOD:The cell-count in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by the wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed using SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α), IL-1β, and IL-6 were assayed using an enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed through HE staining. The inflammatory signal pathway related protein nuclear factor NF-κB p65 mRNA expression was measured by real-time PCR, and the protein level of NF-κB p65 was measured using Western blotting analysis.
RESULTS:The data showed that treatment with the tectorigenin markedly attenuated the inflammatory cell numbers in the BALF, decreased nuclear factor NF-κB p65 mRNA level and protein level in the lungs, and improved SOD activity and inhibited MPO activity. Histological studies showed that tectorigenin substantially inhibited LPS-induced neutrophils in lung tissue compared with the model group.
CONCLUSION:The results indicated that tectorigenin had a protective effect on LPS-induced ALI in mice.
