Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application.
10.1016/S1875-5364(14)60127-8
- Author:
Yuan TAO
1
;
Jun LIN
2
;
You-Jia HU
1
;
Bin ZHOU
1
;
Bao-Quan ZHU
3
Author Information
1. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China.
2. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China. Electronic address: lin.jun@sipi.com.cn.
3. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China. Electronic address: baoquanzhu@sipi.com.cn.
- Publication Type:Journal Article
- Keywords:
ELISA kit;
Human Secreted CD306/LAIR-2;
Monoclonal antibody;
Polyclonal antibody
- MeSH:
Animals;
Antibodies, Monoclonal;
immunology;
Enzyme-Linked Immunosorbent Assay;
instrumentation;
methods;
Humans;
Mice;
Mice, Inbred BALB C;
Rabbits;
Receptors, Immunologic;
analysis;
blood;
immunology;
Recombinant Proteins
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(11):847-852
- CountryChina
- Language:English
-
Abstract:
AIM:A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.
METHODS:Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.
RESULTS:A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.
CONCLUSIONS:The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.
