Methanolic extract of Momordica cymbalaria enhances glucose uptake in L6 myotubes in vitro by up-regulating PPAR-γ and GLUT-4.
10.1016/S1875-5364(14)60132-1
- Author:
Puttanarasaiah Mahesh KUMAR
1
;
Marikunte V VENKATARANGANNA
2
;
Kirangadur MANJUNATH
3
;
Gollapalle L VISWANATHA
4
;
Godavarthi ASHOK
5
Author Information
1. Padmashree Institute of Information Sciences, Bangalore 560072, India. Electronic address: maheshguni@yahoo.com.
2. Connexios Life Sciences Pvt Ltd., J.P. Nagar, Bangalore 560078, India.
3. Department of Microbiology and Biotechnology, Bangalore University, Bangalore 560056, India.
4. PES College of Pharmacy, Bangalore 560050, India.
5. Director, Radiant Research Services Pvt Ltd., Bangalore 560050, India.
- Publication Type:Journal Article
- Keywords:
Antidiabetic activity;
Diabetes;
Glucose transport;
L6 myotubes;
Momordica cymbalaria;
PPARγ
- MeSH:
Biological Transport;
Dose-Response Relationship, Drug;
Fruit;
Gene Expression;
drug effects;
Glucose;
metabolism;
Glucose Transporter Type 4;
metabolism;
Hypoglycemic Agents;
pharmacology;
In Vitro Techniques;
Insulin;
metabolism;
Momordica;
Muscle Fibers, Skeletal;
drug effects;
PPAR gamma;
metabolism;
Plant Extracts;
pharmacology;
Protein Biosynthesis;
Protein Synthesis Inhibitors;
pharmacology;
Rosiglitazone;
Thiazolidinediones;
pharmacology;
Up-Regulation
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(12):895-900
- CountryChina
- Language:English
-
Abstract:
The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL(-1) were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL(-1)) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro.
