Ginkgo biloba extracts attenuate lipopolysaccharide-induced inflammatory responses in acute lung injury by inhibiting the COX-2 and NF-κB pathways.
10.1016/S1875-5364(15)60006-1
- Author:
Xin YAO
1
;
Nan CHEN
2
;
Chun-Hua MA
3
;
Jing TAO
1
;
Jian-An BAO
1
;
Zong-Qi CHENG
1
;
Zu-Tao CHEN
4
;
Li-Yan MIAO
5
Author Information
1. Department of Pharmacy, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
2. Medical College of Soochow University, Suzhou 215000, China.
3. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
4. Department of Infectious Diseases, First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: zutaochen@163.com.
5. Department of Pharmacy, First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: miaolysuzhou@163.com.
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase;
Ginkgo biloba extract;
Lipopolysaccharide;
Nuclear factor-κB
- MeSH:
Acute Lung Injury;
chemically induced;
drug therapy;
metabolism;
Animals;
Bronchoalveolar Lavage Fluid;
cytology;
Cell Count;
Cyclooxygenase 2;
genetics;
metabolism;
Enzyme-Linked Immunosorbent Assay;
Gene Expression;
drug effects;
Ginkgo biloba;
chemistry;
Interleukin-1beta;
analysis;
Interleukin-6;
analysis;
Lipopolysaccharides;
Lung;
immunology;
pathology;
Male;
Mice;
Mice, Inbred BALB C;
Peroxidase;
metabolism;
Phytotherapy;
Plant Extracts;
pharmacology;
Pulmonary Edema;
Superoxide Dismutase;
metabolism;
Transcription Factor RelA;
genetics;
metabolism;
Tumor Necrosis Factor-alpha;
analysis
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(1):52-58
- CountryChina
- Language:English
-
Abstract:
In the present study, we analyzed the role of Ginkgo biloba extract in lipopolysaccharide(LPS)-induced acute lung injury (ALI). ALI was induced in mice by intratracheal instillation of LPS. G. biloba extract (12 and 24 mg·kg(-1)) and dexamethasone (2 mg·kg(-1)), as a positive control, were given by i.p. injection. The cells in the bronchoalveolar lavage fluid (BALF) were counted. The degree of animal lung edema was evaluated by measuring the wet/dry weight ratio. The superoxidase dismutase (SOD) and myeloperoxidase (MPO) activities were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators, tumor necrosis factor-a, interleukin-1b, and interleukin-6, were assayed by enzyme-linked immunosorbent assay. Pathological changes of lung tissues were observed by H&E staining. The levels of NF-κB p65 and COX-2 expression were detected by Western blotting. Compared to the LPS group, the treatment with the G. biloba extract at 12 and 24 mg·kg(-1) markedly attenuated the inflammatory cell numbers in the BALF, decreased NF-κB p65 and COX-2 expression, and improved SOD activity, and inhibited MPO activity. The histological changes of the lungs were also significantly improved. The results indicated that G. biloba extract has a protective effect on LPS-induced acute lung injury in mice. The protective mechanism of G. biloba extract may be partly attributed to the inhibition of NF-κB p65 and COX-2 activation.
