Metabolite profiling of Zi-Shen pill in rat biological specimens by UPLC-Q-TOF/MS.
10.1016/S1875-5364(15)60019-X
- Author:
Xiao-Wei LIU
1
;
Feng ZHANG
1
;
Shou-Hong GAO
1
;
Bo JIANG
1
;
Wan-Sheng CHEN
2
Author Information
1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
2. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: chenwanshengsmmu@aliyun.com.
- Publication Type:Journal Article
- Keywords:
Chemical constituents;
Metabolites;
UPLC-Q-TOF/MS;
Zi-Shen pill
- MeSH:
Alkaloids;
blood;
urine;
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
metabolism;
Male;
Plasma;
chemistry;
Rats;
Tandem Mass Spectrometry;
Urine;
chemistry;
Xanthones;
blood;
urine
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(2):145-160
- CountryChina
- Language:English
-
Abstract:
This study aimed to profile the chemical constituents of Zi-Shen pill (ZSP) and its metabolites in plasma, urine, and prostate tissue, after administration into rats. Based on the chromatographic retention behavior, fragmentation patterns of chemical components, published literatures, and literature databases, an UPLC-Q-TOF/MS (LC-TOF/MS) method was established to identify the components of ZSP and its metabolites in biological samples. A total of 101 compounds were identified and tentatively characterized from the ZSP, including alkaloids, xanthones, and timosaponins. Except for 33 prototype components, 22 metabolites were detected in the plasma, urine, and prostate, and mainly came from Phellodendri Amurensis Cortex and Anemarrhenae Rhizoma. It was found that glucuronidation and sulfation were the major metabolic processes of xanthones, while oxidation, demethylation, and glucuronidation were the major metabolic pathways of alkaloids. In summary, the present study provided important chemical information on the metabolism of ZSP, indicating that alkaloids might be able to be absorbed into the prostate. The results provided a basis for further studies of the mechanisms of action for ZSP.
