The polysaccharide isolated from Pleurotus nebrodensis (PN-S) shows immune-stimulating activity in RAW264.7 macrophages.
10.1016/S1875-5364(15)30026-1
- Author:
Hai-Yan CUI
1
;
Chang-Lu WANG
2
;
Yu-Rong WANG
1
;
Zhen-Jing LI
1
;
Ya-Nan ZHANG
1
Author Information
1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
2. Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: clw123@tust.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cytokines;
Immunization;
Pleurotus nebrodensis;
Polysaccharide
- MeSH:
Animals;
Cell Cycle;
immunology;
Cell Line;
Cell Proliferation;
drug effects;
Fungal Polysaccharides;
pharmacology;
Immunity;
drug effects;
Interferon-gamma;
biosynthesis;
metabolism;
Interleukin-6;
biosynthesis;
metabolism;
Macrophages;
immunology;
metabolism;
Mice;
Nitric Oxide;
biosynthesis;
Nitric Oxide Synthase Type II;
metabolism;
Pleurotus;
RNA, Messenger;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Necrosis Factor-alpha;
biosynthesis;
metabolism;
Up-Regulation
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(5):355-360
- CountryChina
- Language:English
-
Abstract:
A novel Pleurotus nebrodensis polysaccharide (PN-S) was purified and characterized, and its immune-stimulating activity was evaluated in RAW264.7 macrophages. PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner, as determined by the MTT assay. After exposure to PN-S, the phagocytosis of the macrophages was significantly improved, with remarkable changes in morphology being observed. Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases. PN-S treatment enhanced the productions of interleukin-6 (IL-6), nitric oxide (NO), interferon gamma (INF-γ), and tumor necrosis factor-α (TNF-α) in the macrophages, with up-regulation of mRNA expressions of interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), interferon gamma(INF-γ) and tumor necrosis factor-α (TNF-α) being observed in a dose-dependent manner, as measured by qRT-PCR. In conclusion, these results suggest that the purified PN-S can improve immunity by activating macrophages.
