Pharmacokinetics and correlation between in vitro release and in vivo absorption of bio-adhesive pellets of panax notoginseng saponins.
10.1016/S1875-5364(17)30029-8
- Author:
Ying LI
1
;
Yun ZHANG
1
;
Chun-Yan ZHU
2
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. Electronic address: cyzhu@implad.ac.cn.
- Publication Type:Journal Article
- Keywords:
Bio-adhesive pellets;
In vivo and in vitro correlation;
Panax notoginseng saponins;
Pharmacokinetics
- MeSH:
Acrylic Resins;
Adhesives;
Animals;
Chitosan;
Drug Carriers;
Drug Liberation;
In Vitro Techniques;
Intestinal Absorption;
Male;
Methylcellulose;
Panax notoginseng;
chemistry;
Plant Extracts;
administration & dosage;
metabolism;
pharmacokinetics;
Rats, Sprague-Dawley;
Saponins;
administration & dosage;
metabolism;
pharmacokinetics
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(2):142-151
- CountryChina
- Language:English
-
Abstract:
The present study was designed to prepare and compare bio-adhesive pellets of panax notoginseng saponins (PNS) with hydroxy propyl methyl cellulose (HPMC), chitosan, and chitosan : carbomer, explore the influence of different bio-adhesive materials on pharmacokinetics behaviors of PNSbio-adhesive pellets, and evaluate the correlation between in vivo absorption and in vitro release (IVIVC). In order to predict the in vivo concentration-time profile by the in vitro release data of bio-adhesive pellets, the release experiment was performed using the rotating basket method in pH 6.8 phosphate buffer. The PNS concentrations in rat plasma were analyzed by HPLC-MS-MS method and the relative bioavailability and other pharmacokinetic parameters were estimated using Kinetica4.4 pharmacokinetic software. Numerical deconvolution method was used to evaluate IVIVC. Our results indicated that, compared with ordinary pellets, PNS bio-adhesive pellets showed increased oral bioavailability by 1.45 to 3.20 times, increased C, and extended MRT. What's more, the release behavior of drug in HPMC pellets was shown to follow a Fickian diffusion mechanism, a synergetic function of diffusion and skeleton corrosion. The in vitro release and the in vivo biological activity had a good correlation, demonstrating that the PNS bio-adhesive pellets had a better sustained release. Numerical deconvolution technique showed the advantage in evaluation of IVIVC for self-designed bio-adhesive pellets with HPMC. In conclusion, the in vitro release data of bio-adhesive pellets with HPMC can predict its concentration-time profile in vivo.
