In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice.
10.1016/S1875-5364(17)30032-8
- Author:
Ya-Qun FANG
1
;
Chuan-Bin SHEN
2
,
3
;
Ning LUAN
1
;
Hui-Min YAO
1
;
Chen-Bo LONG
1
;
Ren LAI
4
;
Xiu-Wen YAN
5
Author Information
1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
2. Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
3. University of Chinese Academy of Sciences, Beijing 100009, China.
4. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: rlai72@njau.edu.cn.
5. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: yanxw@njau.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cytokine;
Hepcidin;
Inflammation;
Iron;
Malaria
- MeSH:
Animals;
Antimalarials;
chemical synthesis;
pharmacology;
Disease Models, Animal;
Drug Evaluation, Preclinical;
Hepcidins;
chemical synthesis;
pharmacology;
Humans;
Interleukin-10;
immunology;
Interleukin-17;
immunology;
Malaria;
drug therapy;
immunology;
mortality;
parasitology;
Male;
Mice;
Plasmodium berghei;
drug effects;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(3):161-167
- CountryChina
- Language:English
-
Abstract:
The present study was designed to investigate the antimalarial activity of synthetic hepcidin and its effect on cytokine secretion in mice infected with Plasmodium berghei. The mice were infected with P. berghei intravenously and treated with hepcidin according to 4-day suppression test and Rane's test. The serum levels of interleukins (IL-1β, IL-2, IL-6, IL-10, IL-12p70, and IL-17A), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in the experimental mice were determined using a cytometric bead array (CBA) kit. The survival rate of the infected mice was also registered. Additionally, the serum iron, alanine transaminase (ALT), aspartate transaminase (AST), and total bilirubin (BIL) were detected to evaluate liver functions. Hepcidin exerted direct anti-malarial function in vivo and increased survival rate in a dose-dependent manner. In addition, the secretion of T helper cell type 1 (Th1), Th2, and Th17 cytokines, TNF-α, and IFN-γ were inhibited by hepcidin. In conclusion, our results demonstrated that synthetic hepcidin exerts in vivo antimalarial activity and possesses anti-inflammatory function, which provides a basis for future design of new derivatives with ideal anti-malarial activity.
