Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats.
10.1016/S1875-5364(17)30043-2
- Author:
Gao-Sheng HU
1
,
2
;
Chong-Gui HUANG
3
;
Yang ZHANG
4
;
Wei XIAO
5
;
Jing-Ming JIA
1
,
6
Author Information
1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
2. Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
3. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
4. The Sixth Traditional Chinese Medicines Factory, Zhongxin Pharmaceutical Group Ltd., Tianjin 300401, China.
5. Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
6. Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China. Electronic address: jmingjia@sina.com.
- Publication Type:Journal Article
- Keywords:
Content determination;
Diuretic activity;
Poria cocos;
Triterpenoids;
Two-stage culture
- MeSH:
Animals;
Biomass;
Diuretics;
pharmacology;
Mycelium;
chemistry;
growth & development;
Rats;
Triterpenes;
metabolism;
Wolfiporia;
chemistry;
growth & development
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(4):265-270
- CountryChina
- Language:English
-
Abstract:
Poria cocos (Schw.) Wolf, an important medicinal and food fungus, is well known in East Asia. Due to growing market demand, long cultivation period, and consumption of pine trunk during cultivation, developing alternative methods for producing P. cocos and/or its active components is of interest. In the present study, the effects of different culture methods on biomass and accumulation of four triterpenoids were investigated. The ethanol extract of fermented mycelium (EFM) was orally administered to rats. Urine output and concentrations of electrolytes (Na, K, and Cl) were measured. Our results showed that mycelia grew better under continuous shaking culture condition (7.5 g DW·L), and higher triterpenoid levels were accumulated in two-stage culture (112 mg·L, 2.03%). The optimal starting time of static culture for triterpenoid yield was 4 d after shaking culture. Single administration of middle and high dose of EFM significantly increased urine output, Na and Cl excretion, and Na/K ratio. These results suggested that ethanol extract of cultured mycelia showed significant diuretic activity in rats and two-stage culture of P. cocos could be an alternative way to produce mycelia and triterpenoids.
