Survey of traditional Dai medicine reveals species confusion and potential safety concerns: a case study on Radix Clerodendri Japonicum.
10.1016/S1875-5364(17)30063-8
- Author:
Bao-Zhong DUAN
1
,
2
,
3
;
Hai-Lan FANG
3
,
4
;
Xi-Wen LI
1
,
5
;
Lin-Fang HUANG
6
;
Wang PING
7
;
Shi-Lin CHEN
1
,
8
Author Information
1. College of Pharmaceutical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. College of Pharmaceutical Sciences, Dali University, Dali 671000, China.
4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
5. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: xwli@icmm.ac.cn.
6. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
7. College of Pharmaceutical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China.
8. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: slchen@icmm.ac.cn.
- Publication Type:Journal Article
- Keywords:
Adulterants;
DNA barcode;
Dai medicine;
ITS 2;
Radix Clerodendri Japonicum
- MeSH:
Clerodendrum;
classification;
genetics;
DNA Barcoding, Taxonomic;
Drug Contamination;
Medicine, Chinese Traditional;
adverse effects;
Polymerase Chain Reaction
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(6):417-426
- CountryChina
- Language:English
-
Abstract:
The adulteration of herbal products is a threat to consumer safety. In the present study, we surveyed the species composition of commercial Radix Clerodendri Japonicum products using DNA barcoding as a supervisory method. A reference database for plant-material DNA-barcode was successfully constructed with 48 voucher samples from 12 Clerodendrum species. The database was used to identify 27 Radix Clerodendri Japonicum decoction piece samples purchased from drug stores and hospitals. The DNA sequencing results revealed that only 1 decoction piece (3.70%) was authentic C. japonicum, as recorded in the Dai Pharmacopeia, whereas the other samples were all adulterants, indicating a potential safety issue. The results indicate that decoction pieces that are available in the market have complex origins and that DNA barcoding is a suitable tool for regulation of Dai medicines.
