Enhanced absorption and inhibited metabolism of emodin by 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside: Possible mechanisms for Polygoni Multiflori Radix-induced liver injury.
10.1016/S1875-5364(17)30067-5
- Author:
Qiong YU
1
;
Li-Long JIANG
1
;
Na LUO
1
;
Ya-Xi FAN
1
;
Jiang MA
1
;
Ping LI
1
;
Hui-Jun LI
2
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: cpuli@163.com.
- Publication Type:Journal Article
- Keywords:
2, 3, 5, 4′-Tetrahydroxystilbene-2-O-β-D-glucopyranoside;
Component interaction;
Emodin;
Hepatotoxicity;
Polygoni Multiflori Radix
- MeSH:
Caco-2 Cells;
Chemical and Drug Induced Liver Injury;
etiology;
Emodin;
analysis;
metabolism;
Fallopia multiflora;
adverse effects;
Glucosides;
toxicity;
Glucuronosyltransferase;
antagonists & inhibitors;
Humans;
Plant Roots;
Stilbenes;
toxicity
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(6):451-457
- CountryChina
- Language:English
-
Abstract:
Polygoni Multiflori Radix (PMR) has been commonly used as a tonic in China for centuries. However, PMR-associated hepatotoxicity is becoming a safety issue. In our previous in vivo study, an interaction between stilbenes and anthraquinones has been discovered and a hypothesis is proposed that the interaction between stilbene glucoside-enriching fraction and emodin may contribute to the side effects of PMR. To further support our previous in vivo results in rats, the present in vitro study was designed to evaluate the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside (TSG) on the cellular absorption and human liver microsome metabolism of emodin. The obtained results indicated that the absorption of emodin in Caco-2 cells was enhanced and the metabolism of emodin in human liver microsomes was inhibited after TSG treatment. The effects of the transport inhibitors on the cellular emodin accumulation were also examined. Western blot assay suggested that the depressed metabolism of emodin could be attributed to the down-regulation of UDP-glucuronosyltransferases (UGTs) 1A8, 1A10, and 2B7. These findings definitively demonstrated the existence of interaction between TSG and emodin, which provide a basis for a better understanding of the underlying mechanism for PMR-induced liver injury.
