UPLC/ESI-QTOF-MS-based metabolomics survey on the toxicity of triptolide and detoxication of licorice.
10.1016/S1875-5364(17)30071-7
- Author:
Zhuo WANG
1
,
2
;
Jian-Qun LIU
3
;
Jin-Di XU
4
;
He ZHU
4
;
Ming KONG
4
;
Guo-Hua ZHANG
3
;
Su-Min DUAN
4
;
Xiu-Yang LI
4
;
Guang-Fu LI
5
;
Li-Fang LIU
6
;
Song-Lin LI
7
Author Information
1. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China
2. State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis, China Pharmaceutical University, Nanjing 210009, China.
3. Key Laboratory of Modern Preparation of Traditional Chinese Medicines, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
4. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
5. Department of Surgery, Medical University of South Carolina, Charleston, SC 29466, USA.
6. State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liulifang69@126.com.
7. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China. Electronic address: songlinli64@126.com.
- Publication Type:Journal Article
- Keywords:
Detoxication;
Licorice;
Metabolomics;
Toxicity;
Triptolide;
UPLC/ESI-QTOF-MS
- MeSH:
Animals;
Biomarkers;
Chromatography, High Pressure Liquid;
methods;
Diterpenes;
toxicity;
Epoxy Compounds;
toxicity;
Glycyrrhiza;
Male;
Metabolomics;
Phenanthrenes;
toxicity;
Plant Extracts;
therapeutic use;
Principal Component Analysis;
Rats;
Rats, Sprague-Dawley;
Spectrometry, Mass, Electrospray Ionization;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(6):474-480
- CountryChina
- Language:English
-
Abstract:
Triptolide (TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but its clinical application is confined by intense side effects. It is reported that licorice can obviously reduce the toxicity of TP, but the detailed mechanisms involved have not been comprehensively investigated. The current study aimed to explore metabolomics characteristics of the toxic reaction induced by TP and the intervention effect of licorice water extraction (LWE) against such toxicity. Obtained urine samples from control, TP and TP + LWE treated rats were analyzed by UPLC/ESI-QTOF-MS. The metabolic profiles of the control and the TP group were well differentiated by the principal component analysis and orthogonal partial least squares-discriminant analysis. The toxicity of TP was demonstrated to be evolving along with the exposure time of TP. Eight potential biomarkers related to TP toxicity were successfully identified in urine samples. Furthermore, LWE treatment could attenuate the change in six of the eight identified biomarkers. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan, pantothenic acid, and porphyrin metabolism. Therefore, it was concluded that LWE demonstrated interventional effects on TP toxicity through regulation of tryptophan, pantothenic acid, and porphyrin metabolism pathways, which provided novel insights into the possible mechanisms of TP toxicity as well as the potential therapeutic effects of LWE against such toxicity.
