Polymyxin B as an inhibitor of lipopolysaccharides contamination of herb crude polysaccharides in mononuclear cells.
10.1016/S1875-5364(17)30074-2
- Author:
Xiao-Xiao LU
1
;
Yi-Fan JIANG
1
;
Hong LI
1
;
Ying-Ye OU
1
;
Zhi-De ZHANG
1
;
Hong-Ye DI
2
;
Dao-Feng CHEN
3
;
Yun-Yi ZHANG
4
Author Information
1. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China.
2. Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.
3. Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: dfchen@shmu.edu.cn.
4. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: zhcheng@shmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Bupleurum chinensis polysaccharides;
Lipopolysaccharide;
Mouse peritoneal macrophages;
Polymyxin B;
RAW264.7 cells
- MeSH:
Animals;
Bupleurum;
chemistry;
Drug Contamination;
Drugs, Chinese Herbal;
analysis;
pharmacology;
Lipopolysaccharides;
analysis;
antagonists & inhibitors;
Macrophages;
drug effects;
metabolism;
Mice;
Nitric Oxide;
metabolism;
Polymyxin B;
analysis;
pharmacology;
Polysaccharides;
analysis;
pharmacology;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(7):487-494
- CountryChina
- Language:English
-
Abstract:
Lipopolysaccharides (LPS) contamination in herbal crude polysaccharides is inevitable. The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells. LPS was pretreated with polymyxin B sulfate (PB) at different concentrations for 1, 5 or 24 h, and then used to stimulate RAW264.7 and mouse peritoneal macrophages (MPMs). The nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in cell culture supernatant, as the indications of cell response, were assayed. Bupleurum chinensis polysaccharides (BCPs) with trace amount contamination of LPS was treated with PB. 30 μg·mL of PB, treating LPS (10 and 1 000 ng·mL in stimulating RAW264.7 and MPMs respectively) at 37 °C for 24 h, successfully abolished the stimulating effect of LPS on the cells. When the cells were stimulated with LPS, BCPs further promoted NO production. However, pretreated with PB, BCPs showed a suppression of NO production in MPMs and no change in RAW264.7. In the in vitro experiments, LPS contamination in polysaccharide might bring a great interference in assessing the activity of drug. Pretreatment with PB (30 μg·mL) at 37 °C for 24 h was sufficient to abolish the effects of LPS contamination (10 and 1 000 ng·mL).
