An UHPLC-MS/MS method for simultaneous determination of quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, bilobalide and ligustrazine in rat plasma, and its application to pharmacokinetic study of Xingxiong injection.
10.1016/S1875-5364(17)30101-2
- Author:
Li-Li DOU
1
;
Li DUAN
1
;
Long GUO
1
;
Le-Le LIU
1
;
Yu-Dong ZHANG
2
;
Ping LI
1
;
E-Hu LIU
3
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. Holdwarm Pharmaceutical China Co. Ltd., Nanjing 210008, China.
3. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liuehu2011@163.com.
- Publication Type:Journal Article
- Keywords:
Pharmacokinetics;
Rat plasma;
UHPLC-MS/MS;
Xingxiong injection
- MeSH:
Animals;
Bilobalides;
blood;
pharmacokinetics;
Chromatography, High Pressure Liquid;
methods;
Disaccharides;
blood;
pharmacokinetics;
Drugs, Chinese Herbal;
administration & dosage;
analysis;
pharmacokinetics;
Flavonoids;
blood;
pharmacokinetics;
Glucosides;
blood;
pharmacokinetics;
Kaempferols;
blood;
pharmacokinetics;
Pyrazines;
blood;
pharmacokinetics;
Quercetin;
analogs & derivatives;
blood;
pharmacokinetics;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(9):710-720
- CountryChina
- Language:English
-
Abstract:
The present study was designed to develop and validate a rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of five major active constituents in the traditional Chinese medicinal preparation Xingxiong injection (XXI) in rat plasma, including quercetin 3-O-rutinoside (QCR), kaempferol 3-O-rutinoside (KFR), isorhamnetin 3-O-rutinoside (ISR), bilobalide (BB), and ligustrazine (LGT). The plasma samples were pretreated by protein precipitation with acetonitrile. The chromatographic separation was achieved on a Waters Symmetry C analytical column (2.1 mm × 100 mm, 3.5 μm) with a mobile phase of 0.1% aqueous formic acid (A)-acetonitrile (B). Quantitation of the five bioactive constituents was achieved. Naringin was used as the internal standard (IS). All the calibration curves showed good linearity (r > 0.996) over the concentration range, with the lowest limit of quantification (LLOQ) between 2-18 ng·mL. The intra- and inter-day accuracy and precision of the analytes were both within acceptable limits. Moreover, satisfactory extraction recoveries (90.92%-104.03%) were obtained by protein precipitation. The validated method was successfully applied to a pharmacokinetic study of XXI in rats after intravenous administration at three doses. The pharmacokinetic parameters of the five compounds varied in a dose-dependent manner within the tested dosage range. The present study was the first report of pharmacokinetic study for XXI.
