Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities.
10.1016/S1875-5364(18)30015-3
- Author:
Jia XIONG
1
,
2
;
Mary H GRACE
3
;
Debora ESPOSITO
3
;
Slavko KOMARNYTSKY
3
;
Fei WANG
4
;
Mary Ann LILA
5
Author Information
1. Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China
2. Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
3. Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
4. Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China.
5. Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA. Electronic address: mlila@ncsu.edu.
- Publication Type:Journal Article
- Keywords:
Acacia mearnsii;
Anti-inflammatory;
Proanthocyanidins;
α-Amylase;
α-Glucosidase
- MeSH:
Acacia;
chemistry;
Animals;
Anti-Inflammatory Agents;
isolation & purification;
pharmacology;
Carbohydrate Metabolism;
drug effects;
Glycoside Hydrolase Inhibitors;
pharmacology;
Inflammation;
metabolism;
Interleukin-1beta;
metabolism;
Lipopolysaccharides;
Macrophages;
drug effects;
Mice;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
metabolism;
Plant Bark;
chemistry;
Plant Extracts;
chemistry;
pharmacology;
Polyphenols;
isolation & purification;
pharmacology;
Proanthocyanidins;
pharmacology;
RAW 264.7 Cells;
alpha-Amylases;
antagonists & inhibitors;
alpha-Glucosidases;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(11):816-824
- CountryChina
- Language:English
-
Abstract:
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC of 0.4-1.4 μg·mL) than the pharmaceutical acarbose (IC 141.8 μg·mL). B6 and B7 (IC 17.6 and 11.7 μg·mL, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC 15.4 μg·mL). Moreover, B extract, at 25 µg·mL, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
