Early intervention with Didang decoction delays macrovascular lesions in diabetic rats through regulating AMP-activated protein kinase signaling pathway.
10.1016/S1875-5364(18)30018-9
- Author:
Dan-Dan REN
1
,
2
;
Jing LI
3
;
Bai CHANG
4
;
Chun-Shen LI
5
;
Ju-Hong YANG
3
Author Information
1. Metabolic Diseases Hospital, Tianjin Medical University, Tianjin 300070, China
2. Tanggu Dahua Hospital, Tianjin Coastal New Area, Tianjin 300455, China.
3. Metabolic Diseases Hospital, Tianjin Medical University, Tianjin 300070, China.
4. Metabolic Diseases Hospital, Tianjin Medical University, Tianjin 300070, China. Electronic address: changbai1972@126.com.
5. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. Electronic address: lichunshen@126.com.
- Publication Type:Journal Article
- Keywords:
AMPK;
Caspase-3;
Didang decoction;
Endothelial cells;
PGC-1α
- MeSH:
AMP-Activated Protein Kinases;
metabolism;
Adenosine Triphosphate;
metabolism;
Animals;
Aorta;
drug effects;
metabolism;
Cardiovascular Diseases;
metabolism;
prevention & control;
Caspase 3;
metabolism;
Diabetes Mellitus, Experimental;
complications;
drug therapy;
metabolism;
Diabetes Mellitus, Type 2;
complications;
drug therapy;
metabolism;
Diptera;
Drugs, Chinese Herbal;
pharmacology;
therapeutic use;
Endothelial Cells;
drug effects;
metabolism;
Endothelium, Vascular;
drug effects;
metabolism;
Energy Metabolism;
drug effects;
Leeches;
Mitochondria;
drug effects;
metabolism;
Nitric Oxide Synthase Type III;
metabolism;
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha;
metabolism;
Phytotherapy;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Prunus persica;
Rats, Sprague-Dawley;
Rheum;
Signal Transduction
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(11):847-854
- CountryChina
- Language:English
-
Abstract:
The study aimed to investigate the intervening role of Didang decoction (DDD) at different times in macrovascular endothelial defense function, focusing on its effects on the AMP-activated protein kinase (AMPK) signaling pathway. The effects of DDD on mitochondrial energy metabolism were also investigated in rat aortic endothelial cells (RAECs). Type 2 diabetes were induced in rats by streptozotocin (STZ) combined with high fat diet. Rats were randomly divided into non-intervention group, metformin group, simvastatin group, and early-, middle-, late-stage DDD groups. Normal rats were used as control. All the rats received 12 weeks of intervention or control treatment. Western blots were used to detect the expression of AMP-activated protein kinase α1 (AMPKα1) and peroxisome proliferator-activated receptor 1α (PGC-1α). Changes in the intracellular AMP and ATP levels were detected with ELISA. Real-time-PCR was used to detect the mRNA level of caspase-3, endothelial nitric oxide synthase (eNOS), and Bcl-2. Compared to the diabetic non-intervention group, a significant increase in the expression of AMPKα1 and PGC-1α were observed in the early-stage, middle-stage DDD groups and simvastatin group (P < 0.05). The levels of Bcl-2, eNOS, and ATP were significantly increased (P < 0.05), while the level of AMP and caspase-3 were decreased (P < 0.05) in the early-stage DDD group and simvastatin group. Early intervention with DDD enhances mitochondrial energy metabolism by regulating the AMPK signaling pathway and therefore may play a role in strengthening the defense function of large vascular endothelial cells and postpone the development of macrovascular diseases in diabetes.
