HPPR encodes the hydroxyphenylpyruvate reductase required for the biosynthesis of hydrophilic phenolic acids in Salvia miltiorrhiza.
10.1016/S1875-5364(18)30008-6
- Author:
Guo-Quan WANG
1
;
Jun-Feng CHEN
2
,
3
;
Bo YI
4
;
He-Xin TAN
5
,
6
;
Lei ZHANG
5
,
7
;
Wan-Sheng CHEN
8
Author Information
1. School of Biomedical Sciences, Huaqiao University, Fujian 362000, China.
2. Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
4. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
5. School of Biomedical Sciences, Huaqiao University, Fujian 362000, China
6. Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: hexintan@163.com.
7. Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: zhanglei@smmu.edu.cn.
8. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: chenws126@126.com.
- Publication Type:Journal Article
- Keywords:
Biosynthesis pathway;
Hydrophilic phenolic acids;
Llithospermic acid B;
Rosmarinic acid;
Salvia miltiorrhiza
- MeSH:
Amino Acid Sequence;
Benzofurans;
Biosynthetic Pathways;
genetics;
Cinnamates;
Depsides;
Gene Expression Regulation, Plant;
genetics;
Oxidoreductases;
genetics;
Phenylpropionates;
metabolism;
Phenylpyruvic Acids;
metabolism;
Phylogeny;
Plant Proteins;
genetics;
metabolism;
Plant Roots;
chemistry;
enzymology;
genetics;
metabolism;
Plants, Genetically Modified;
Recombinant Proteins;
analysis;
biosynthesis;
Salvia miltiorrhiza;
chemistry;
enzymology;
genetics;
metabolism;
Sequence Alignment
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(12):917-927
- CountryChina
- Language:English
-
Abstract:
Salvia miltiorrhiza is a medicinal plant widely used in the treatment of cardiovascular and cerebrovascular diseases. Hydrophilic phenolic acids, including rosmarinic acid (RA) and lithospermic acid B (LAB), are its primary medicinal ingredients. However, the biosynthetic pathway of RA and LAB in S. miltiorrhiza is still poorly understood. In the present study, we accomplished the isolation and characterization of a novel S. miltiorrhiza Hydroxyphenylpyruvate reductase (HPPR) gene, SmHPPR, which plays an important role in the biosynthesis of RA. SmHPPR contained a putative catalytic domain and a NAD(P)H-binding motif. The recombinant SmHPPR enzyme exhibited high HPPR activity, converting 4-hydroxyphenylpyruvic acid (pHPP) to 4-hydroxyphenyllactic acid (pHPL), and exhibited the highest affinity for substrate 4-hydroxyphenylpyruvate. SmHPPR expression could be induced by various treatments, including SA, GA, MeJA and Ag, and the changes in SmHPPR activity were correlated well with hydrophilic phenolic acid accumulation. SmHPPR was localized in cytoplasm, most likely close to the cytosolic NADPH-dependent hydroxypyruvate reductase active in photorespiration. In addition, the transgenic S. miltiorrhiza hairy roots overexpressing SmHPPR exhibited up to 10-fold increases in the products of hydrophilic phenolic acid pathway. In conclusion, our findings provide a new insight into the synthesis of active pharmaceutical compounds at molecular level.
