Screening and identification of MAGE-A11 related genes based on DNAmicroarray
10.3872/j.issn.1007-385x.2018.09.010
- VernacularTitle:基因芯片技术筛查并鉴定乳腺癌细胞中MAGE-A11的相关基因
- Author:
GU Lina
1
;
SANG Meixiang
2
;
LI Juan
1
;
LIU Fei
1
;
WANG Pengyu
1
;
YIN Danjing
1
;
WU Yunyan
1
;
SHAN Baoen
2
Author Information
1. Department of Research Center
2. (a. Department of Research Center；b. Department of Immunology, Tumor Research Institute, the Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
breast cancer;
melanoma antigen-A11（MAGE-A11）;
DNAmicroarray
- From:
Chinese Journal of Cancer Biotherapy
2018;25(9):904-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen related genes of melanoma-associated antigen-A11 (MAGE-A11) in breast cancer cells based on highthroughput DNAmicroarray technology, and to validate from the aspects of quantity and function. Methods: DNAmicroarray was used to screen the differently-expresseddown-stream mRNAs of MAGE-A11 in breast cancercelllines (MCF-7, MDA-MB-231 and BT-549). Cluster analysis was applied on representative genes and quantitative RT-PCR was used to validate. CCK-8, scratch wound healing assay and Transwell assaywere used to detect the effect of MAGE-A11 on the proliferation,migration and invasion of breast cancer cells. Results: Over-expression of MAGE-A11 caused the differential expression of 1608 down-stream genes in 3 breast cancer cell lines, which was associated with various cell functions such as protein ubiquitination,cell proliferation and apoptosis, tumor invasion and metastasis.qRT-PCR validated that the expression of ZNF-451, CENPTJ, CDK13, API5 and LMO7, which were highly expressed in microarray, were also significantly higher than those in control group (P<0.01);in addition, SHPRH, PML, MARK2, LIMA1 and ANGPTL4, which were low-expressed in microarray, were also significantly lower than those in control group (P<0.01). MAGE-A11transfection directly increased the proliferation of breast cancer MCF-7, MDA-MB-231 and BT-549 cells at 72 h (all P<0.01); compared with control group after transfectionexhibited obvious wound healing at 48 h (P<0.05 or P<0.01) and significantly increased trans-membrane cell numbers (all P<0.01). Conclusion: Many differentially expressed genes related to ubiquitination, cell proliferation and apoptosis, tumor invasion and migration were screened in MCF-7, MDA-MB-231 and BT-549 breast cancer cells. Among them, 10 typical differentially expressed genes were identified in terms of quantity and function.
- Full text:201809101.pdf
