Regulatory effect of hsa-miR-150-5p on malignant behaviors of glioblastoma U-251MG cells by targeting HIF1α
10.3872/j.issn.1007-385x.2018.09.006
- VernacularTitle:hsa-miR-150-5p靶向HIF1α对成胶质细胞瘤U-251MG细胞恶性生物学 行为的影响
- Author:
ZHOU Jiang
1
;
XIA Xiangguo
1
;
CHEN Ligang
1
;
LUO Xin
1
;
AKU Buqian
1
;
GUO Zhangchao
1
Author Information
1. (Department of Neurosurgery,Affiliated Hospital of Southwestern Medical University
- Publication Type:Journal Article
- Keywords:
glioblastoma(GBM)；miR-150-5p；hypoxia inducible factor 1α（HIF1α）；invasion;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(9):878-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect and possible mechanisms of has-miR-150-5p targeting HIF1α to regulate malignant biological behaviors of glioblastoma (GBM) U-251MG cells. Methods: Real-time quantitative PCR (RT-PCR) was used to detect the expression of miR-150-5p and hypoxia inducible factor 1 (HIF1α) in U-251MG cells. Luciferase report assay was carried out to verify the biological relationship between miR-150-5p and HIF1α and their biological functions in U-251MG cells. The protein expressions of miR150-5pand HIF1α in U-251MG cells were detected by western blotting. The ability of cell migration was detected by wound healing test and cell invasion ability was detected by transwell test. Results: After miR-150-5p mimic transfection, the mRNA expression of HIF1α was significantly reduced in U-251MG cells (P<0.01). Bioinformatics prediction and luciferase reporter assay demonstrated that miR-150-5p down-regulated HIF1α through directly binding to HIF1α 3’-untranslated region (3’-UTR) (all P<0.05). In U-251MG cells, miR-150-5p over-expression significantly inhibited HIF1α expression, cell invasion and migration (all P<0.05). Conclusion: miR150-5p inhibits cell invasion and metastasis through negative regulation of HIF1α, indicating that miR-150-5p and HIF1α were both potential therapeutic targets for glioblastoma.
- Full text:20180906.pdf
