Identification of major constituents from process residue of tripterygium glycosides by HPLC-QTOF-MS
10.11665/j.issn.1000-5048.20150609
- VernacularTitle:HPLC-QTOF-MS法鉴别雷公藤多苷工艺残渣中的化学成分
- Author:
Mingyang LI
1
;
Jiqin HOU
;
Dandan WEI
;
Zhenzhou JIANG
;
Ming YAN
;
Hao WANG
Author Information
1. 中国药科大学天然药物化学教研室，天然药物活性组分与药效国家重点实验室
- Publication Type:Journal Article
- Keywords:
tripterygium glycosides;
process residue;
HPLC-QTOF-MS;
qualitative analysis
- From:
Journal of China Pharmaceutical University
2015;46(6):689-695
- CountryChina
- Language:Chinese
-
Abstract:
A qualitative analytical method of liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-QTOF-MS)was developed for identification of major constituents in process residue of tripterygium glycosides. The HPLC-QTOF-MS assay was performed on a Zorbax SB-C18 column(4. 6 mm × 50 mm, 1. 8 μm)with the mobile phase consisting of acetonitrile and water containing 0. 2% formic acid in gradient mode. Positive ion mode was used for TOF-MS. According to the accurate molecular weight, MS fragment pathway, comparison with the retention time of reference compounds, total 30 compounds, including fifteen alkaloids, ten diterpenoids, four triterpenoids and an unsaturated fatty acid were identified or tentatively characterized in process residue of tripterygium glycosides. This study may be helpful to the comprehensive exploitation and utilization of process residue of tripterygium glycosides.
