Preparation of an electrochemical immunosensor modified with graphene for detection of dienestrol in pork
10.11665/j.issn.1000-5048.20150608
- VernacularTitle:采用石墨烯修饰的电化学免疫传感器检测猪肉中的己二烯雌酚含量
- Author:
Jie ZHANG
1
;
Jun WU
;
Chuanxian WANG
;
Kefeng SHAO
;
Changyun CHEN
;
Bo ZHAO
Author Information
1. 上海海洋大学食品学院
- Publication Type:Journal Article
- Keywords:
graphene;
electrochemical immunosensor;
indirect competition;
dienestrol;
pork;
determination of content
- From:
Journal of China Pharmaceutical University
2015;46(6):683-688
- CountryChina
- Language:Chinese
-
Abstract:
An ultrasensitive electrochemical immunosensor modified with graphene and dienestrol(DE)was developed for the detection of dienestrol through indirect competition with K3Fe(CN)6 acting as the redox probes. The results revealed that under optimized conditions a calibration for DE was obtained with a linear range of 500-5 000 ng/mL and the detection limit was up to 0. 2 ng/mL, showing that the proposed electrochemical immunosensor had excellent sensitivity and wide detection range. The immunosensor was examined in real samples for the analysis of DE. A good recovery in the range of 83. 8%-97. 7% was obtained in pork samples.
