Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS
10.11665/j.issn.1000-5048.20150607
- VernacularTitle:短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定
- Author:
Jiaquan CHEN
1
;
Hui WANG
;
Zhiyuan ZHANG
;
Xiaoting ZHAI
;
Rong YIN
;
Fenxia ZHU
Author Information
1. 中国药科大学分析化学教研室
- Publication Type:Journal Article
- Keywords:
pumiloside;
preparation;
metabolites;
UPLC-QTOF/MS
- From:
Journal of China Pharmaceutical University
2015;46(6):677-682
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, water extract of Nauclea officinalis was absorbed by AB-8 macroporous resin and subsequently gradient-eluted with alcoholic solution of different proportion to prepare pumiloside monomer of high purity. The metabolites in urine, feces and bile of rats with gavage administration were analyzed by UPLC-QTOF/MS. Accurate MS/MS data of all components were collected with full scan mode, and analyzed by MetaboLynx software. Results showed that the monomer of high-purity pumiloside was prepared and four metabolites in rats were identified.
