Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9
10.11665/j.issn.1000-5048.20150413
- VernacularTitle:黄酮类化合物GL-V9对人胃低分化黏液腺癌细胞株的凋亡作用及其机制
- Author:
Guojun LI
1
;
Kai ZHAO
;
Yuyuan YAO
;
Le SHEN
;
Qinglong GUO
Author Information
1. 中国药科大学江苏省肿瘤发生与干预重点实验室；天然药物活性组分与药效国家重点实验室
- Publication Type:Journal Article
- Keywords:
flavonoid compound;
GL-V9;
gastric adenocarcinoma;
MGC-803 cell;
apoptosis;
Ca2+
- From:
Journal of China Pharmaceutical University
2015;46(4):464-468
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the apoptotic effect of flavonoid compound GL-V9 on human gastric cancer cells and its potential mechanism, MGC-803 and BGC-823 cells were treated with GL-V9. MTT assay was performed to assess the growth inhibition effects on MGC-803 and BGC-823 cells under different concentrations of GL-V9. Annexin V-FITC/PI staining assay was employed to observe the apoptotic rate of GL-V9 cells with the treatment of GL-V9. DAPI staining was performed to observe the nuclear morphological changes using fluorescence microscopy. Activation of caspase-9 and caspase-3 was analyzed by Western blotting. Ca2+ concentration in gastric cancer cells was detected by Fluo-3 AM staining assay. Results showed that GL-V9 could inhibitcell viability, change the nuclear morphologyl, activate caspase-9 and caspase-3 and induce the apoptosis in gastric cancer cells. The mechanism of the induction of apoptosis in MGC-803 cells under the treatment of GL-V9 may aetivate the Ca2+ associated mitochondrial apoptosis pathway.
