Preparation and characterization of nanostructured lipid carriers with difference in particle size
10.11665/j.issn.1000-5048.20150409
- VernacularTitle:粒径差异化的纳米脂质载体的制备和表征
- Author:
Huipeng LI
1
;
Zhigui SU
;
Minglei CHEN
;
MENG MENG
;
Minjie SUN
;
Yan WANG
;
Can ZHANG
;
Qineng PING
Author Information
1. 中国药科大学药剂学教研室
- Publication Type:Journal Article
- Keywords:
coumarin-6;
nanostructured lipid carriers;
physicochemical property;
stability in vitro;
in vivo imaging
- From:
Journal of China Pharmaceutical University
2015;46(4):436-443
- CountryChina
- Language:Chinese
-
Abstract:
To construct nanostructured lipid carriers(NLCs)with different particle sizes but the same other physicochemical properties, central composite design was adopted. Coumarin-6(C-6)was selected as the model drug due to its high lipophilicity and high fluorescence intensity. Physicochemical properties of NLCs with 100 nm, 200 nm and 300 nm in particle size could remain stable during certain time in K-R solution and PBS. Release experiments in vitro showed that cumulative release of C-6 in NLCs was less than 7% after 24 h. The MTT assay indicated that both blank NLCs and C-6 loaded NLCs showed low toxicity. To confirm the integrity of NLCs in gastrointestinal tract, DiR-loaded NLCs were prepared and the distribution in vivo was monitored by fluorescence imaging. After 6 h oral administration, intact DiR-loaded NLCs could stiu be found, suggesting that NLCs could be used to characterize the uptake in gastrointestinal tract.
