Inhibition of tetramethypyrazine on proliferation of HepG2 cells and its effects on the pathway of mitochondrial apoptosis

VernacularTitle:川芎嗪抑制肝癌HepG2细胞增殖及对线粒体凋亡途径的影响
Author: Quanfu FENG ¹ ; Lei BI ; Xiaojing YAN ; Ye YANG ; Weiping CHEN
Author Information

1. 南京中医药大学基础医学院
Publication Type:Journal Article
Keywords: tetramethypyrazine; HepG2; apoptosis; mitochondrion; liver cancer
From: Journal of China Pharmaceutical University 2015;46(3):350-354
CountryChina
Language:Chinese
Abstract: The purpose of the present study was to investigate the anti-proliferative and apoptotic effects of tetramethypyrazine(TMP)on HepG2 and to elucidate the underlying mechanisms. CCK-8 was introduced to analyze the HepG2 cells proliferation. Cell apoptosis, mitochondrial membrane potential(ΔΨm)and cytochrome C were measured by high content screening(HCS). Cleaved-caspases protein expression was detected by Western blot. CCK-8 assay indicated that TMP significantly inhibited HepG2 cells proliferation in dose-dependent manner compared with the control group. Moreover, it was found that TMP could also induce HepG2 cell apoptosis, directly increase the release of cytochrome C, decrease ΔΨm and increase cleaved-caspase-3 and cleaved-caspase-9 protein expression. TMP may inhibit cell proliferation and induce cell apoptosis by stimulating the mitochondrial pathway apoptosis in HepG2 cells.