Tetramethylpyrazine attenuates morphine tolerance through suppressing spinal microglia activation in mice

VernacularTitle:川芎嗪通过抑制脊髓小胶质细胞活化缓解吗啡耐受
Author: Lu CHEN ¹ ; Jiajie LI ; Cailong PAN ; Danli ZHOU ; Wentao LIU ; Guangqin ZHANG
Author Information

1. 中国药科大学临床药学教研室
Publication Type:Journal Article
Keywords: tetramethylpyrazine; morphine; chronic tolerance; microglia
From: Journal of China Pharmaceutical University 2015;46(2):230-234
CountryChina
Language:Chinese
Abstract: The aim of the present study was to investigate the effects and possible mechanism of tetramethylpyrazine(TMP)on morphine-induced microglia activation and tolerance. The antinociception and morphine tolerance were assessed in mice using hot-water tail flick test. IBA-1(ionized calcium binding adapter molecule 1), the marker of microglia, was detected by immumofluorescence method. The expression of p-p38 MAPK and total p38 MAPK(mitogen-activated protein kinase, MAPK)was analyzed by Western blot; real-time polymerase chain reaction(RT-PCR)was used to detect the expression level of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β). Results showed that TMP(15, 30, 60 mg/kg, ip)inhibited morphine-induced up-regulation of IBA-1, p-p38, TNF-α and IL-1β in a dose-dependent manner, yet with no effect on the expression of total p38 MAPK. In conclusion, TMP significantly inhibited the activation of microglia evoked by morphine via p38 MAPK signaling pathway, thus attenuating morphine antinociception tolerance.