Mass spectrometry-based protein quantification and its application in pharmacokinetic research
10.11665/j.issn.1000-5048.20150201
- VernacularTitle:基于生物质谱的蛋白质定量技术及其在药代动力学研究中的应用
- Author:
Mengqiu Wu
1
;
Mengqiu WU
;
Gaoyuan LU
;
Chang SHAO
;
Dandan WANG
;
Di SUN
;
Haiping HAO
;
Guangji WANG
Author Information
1. 中国药科大学药物代谢动力学重点实验室
- Publication Type:Journal Article
- Keywords:
biological mass spectrometry;
protein quantification;
drug transporters;
metabolizing enzymes;
therapeutic peptides and proteins
- From:
Journal of China Pharmaceutical University
2015;46(2):129-140
- CountryChina
- Language:Chinese
-
Abstract:
Quantitative proteomics is a mass spectrometry-based toolkit used to analyze and quantify entire proteins contained in whole cells, tissues or organisms. It has become an increasingly important element in exploring the mechanism of various biological processes such as discovering novel biomarkers and unknown drug targets. Emerging advances in biological mass spectrometry instrumentation and data acquisition methodologies have provided a state-of-the-art platform for protein quantification, prompting the research of proteomics evolving from the simple qualitative to the accurate quantitative approach. This review aims to introduce the most recent advancements in mass spectrometry instrumentation and methodologies of data acquisition, focusing on their characteristics and applying fields. It also highlights several significant applications of biological mass spectrometry in pharmaceutical research such as quantifitation of drug transporters and metabolizing enzymes, and pharmacokinetic study of therapeutic peptides and proteins.
